| 高氮磷比与酸化共同作用对微小亚历山大藻生长和产毒的影响 |
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| 引用本文： | 郝爽,刘丽君,陈军辉,何秀平,连子如.高氮磷比与酸化共同作用对微小亚历山大藻生长和产毒的影响[J].海洋科学,2021,45(2):1-10. |
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| 作者姓名： | 郝爽 刘丽君 陈军辉 何秀平 连子如 |
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| 作者单位： | 山东大学 海洋学院, 山东 威海 264209;自然资源部第一海洋研究所, 自然资源部海洋生态环境科学与技术重点实验室, 山东 青岛 266061;山东大学 海洋学院, 山东 威海 264209;自然资源部第一海洋研究所, 自然资源部海洋生态环境科学与技术重点实验室, 山东 青岛 266061 |
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| 基金项目： | 山东省自然科学基金（ZR2019MD026）；国家基金委-山东省联合基金（U1806202）；中国科学院海洋生态与环境科学重点实验室（中国科学院海洋研究所）开放基金（KLMEES202003） |
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| 摘 要： | 通过实验室构建不同氮磷比条件下的酸化海水环境,培养麻痹性贝毒(paralytic shellfish toxin,PST)产毒藻微小亚历山大藻(Alexandrium minutum),采用藻细胞计数法、分光光度法、高效液相色谱-质谱法等分析方法,探讨了不同氮磷比条件下的酸化海水对微小亚历山大藻的生长、氮磷营养盐吸收、产毒等参数的影响。结果表明,在一定程度上,酸化环境对A.minutum的生长、PST的胞内累积和胞外释放有促进作用,且胞外培养液中PST含量增加的程度更高;同时,酸化海水促进胞内毒性较低的N-H类膝沟藻毒素(GTX2和GTX3)被氧化成毒性较高的N-OH类膝沟藻毒素(GTX1和GTX4),导致A.minutum的细胞毒性和培养液毒性增加。而海水中氮磷比的进一步升高,在一定程度上促进了微小亚历山大藻细胞毒素含量和毒性的增加,但并未促进其生长,且降低了藻细胞内的磷储量。
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| 关 键 词： | 氮磷比 酸化环境 微小亚历山大藻 麻痹性贝毒素 |
| 收稿时间： | 2020/9/9 0:00:00 |
| 修稿时间： | 2020/11/13 0:00:00 |
Effects of acidification and high N/P ratios on toxin production in Alexandrium minutum |
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| HAO Shuang,LIU Li-jun,CHEN Jun-hui,HE Xiu-ping,LIAN Zi-ru.Effects of acidification and high N/P ratios on toxin production in Alexandrium minutum[J].Marine Sciences,2021,45(2):1-10. |
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| Authors: | HAO Shuang LIU Li-jun CHEN Jun-hui HE Xiu-ping LIAN Zi-ru |
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| Institution: | Shandong University,Marine College,Shandong University,The First Institute of Oceanography, Ministry of Natural Resources,The First Institute of Oceanography, Ministry of Natural Resources,Marine College,Shandong University |
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| Abstract: | In this paper, the paralytic shellfish toxin (PST) toxin-producing algae were cultivated by laboratory simulated seawater eutrophication and acidification conditions, using algae cell counting method, spectrophotometry, HPLC-MS and other analytical methods. To explore the effects of seawater acidification environment under different N/P ratios on Alexandrium minutum growth, nitrogen and phosphorus nutrient absorption, toxicity and other parameters. The results showed that the acidified seawater promoted the growth and PST intracellular accumulation and extracellular release of A. minutum to a certain extent, and the extracellular PST content increased more. Meanwhile, acidification promoted the oxidation of less toxic N-H gonyautoxin (GTX2 and GTX3) into more toxic N-OH gonyautoxin (GTX1 and GTX4), leading to increased cytotoxicity of A. minutum and toxicity in culture medium. The increased N/P ratios of seawater was beneficial to increase the toxin content and toxicity of A. minutum to a certain extent but did not promote the growth of A. minutum and decreased the intracellular phosphorus reserves. |
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| Keywords: | acidification N/P ratios Alexandrium minutum PST |
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