Comparison of bromodeoxyuridine immunoassay with tritiated thymidine radioassay for measuring bacterial productivity in oceanic waters |
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| Authors: | Koji Hamasaki |
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| Institution: | (1) Graduate School of Biosphere Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan;(2) Present address: Ocean Research Institute, The University of Tokyo, Minamidai, Nakano-ku, Tokyo 164-8639, Japan |
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| Abstract: | Recently, bromodeoxyuridine (BrdU) has been successfully applied to the measurement of bacterial productivity as an alternative
to tritiated thymidine (3H-TdR), which is widely used but often restricted by regulations, particularly in field settings. Here, I report improvements
to existing BrdU methods to simplify procedures and increase sensitivity. The feasibility of the method was tested measuring
bacterial production in low-productive waters. The method provided radioisotope-free measurements of bacterial production
rates at shorter (∼1 h) on-board processing time of samples than previously reported procedures. It was applicable to the
detection of rates ranging from 0.021 to 2.7 pmol BrdU l−1h−1. BrdU incorporation rates measured by immunoassay showed a statistically significant correlation with 3H-TdR incorporation rates measured by radioassay (r = 0.74, n = 24, p < 0.001). The linear regression obtained (BrdU = 0.803H-TdR] − 0.016) showed a similar relationship to previously reported regressions (BrdU = 0.653H-TdR] + 0.12, 3H-BrdU] = 0.693H-TdR] − 0.81). There were no statistically significant differences among these regression lines. These results suggest that
the method described here provides a non-radioisotopic productivity measurement of bacteria in oceanic epipelagic waters,
while retaining continuity of the data with other existing 3H-TdR and BrdU methods. |
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| Keywords: | Bacterial production thymidine bromodeoxyuridine |
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