| Ca2+、Mg2+及盐度对凡纳滨对虾体内代谢酶的影响 |
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| 引用本文： | 张立田,戴习林,臧维玲.Ca2+、Mg2+及盐度对凡纳滨对虾体内代谢酶的影响[J].海洋科学,2013,37(7):63-71. |
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| 作者姓名： | 张立田 戴习林 臧维玲 |
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| 作者单位： | 1. 上海海洋大学 水产与生命学院，上海201306; 东海水产研究所，上海200090
2. 上海海洋大学 水产与生命学院,上海,201306 |
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| 基金项目： | 上海市科技兴农重点攻关项目(沪农科攻字(2010)第1-6 号);上海市科技兴农科技推广项目(沪农科推字(2008)第5-1 号); 上海市教育委员会重点学科建设项目(J50701) |
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| 摘 要： | 为探讨Ca2+、Mg2+、盐度对凡纳滨对虾体内代谢酶的相对独立作用和相互影响,进而为提高凡纳滨对虾生长力和免疫力提供理论依据,本实验采取L49(78)安排7水平Ca2+、Mg2+、盐度,L8(27)安排2水平Ca2+、Mg2+、盐度,开展60 d凡纳滨对虾(Litopenaeus vannamei)养殖试验,通过比较对虾体内消化酶、ATP酶及免疫类酶的活性以分析Ca2+、Mg2+、盐度对凡纳滨对虾生长力和免疫力的影响。结果表明:水体中Ca2+、Mg2+、盐度对消化酶具有显著影响(P<0.05),其中与对虾消化吸收联系最紧密的蛋白酶中,盐度对胃蛋白酶影响显著,盐度为10时酶活最高,Ca2+、盐度对类胰蛋白酶影响显著,Ca2+为200 mg/L,盐度为20时,酶活最高;Ca2+、Mg2+、盐度对ATP酶具有显著影响(P<0.05),其中对Na+-K+-ATP酶都有显著影响,Ca2+为300 mg/L,Mg2+为500 mg/L,盐度为30时酶活最高,Ca2+、Mg2+对Mg2+-ATP酶具有显著影响,Ca2+为200 mg/L,Mg2+为500 mg/L时酶活最高,Ca2+对Ca2+-ATP酶具有显著影响,Ca2+为200、300 mg/L时酶活最高;Ca2+、Mg2+、盐度对凡纳滨对虾体内免疫酶具有显著影响(P<0.05),Ca2+、Mg2+、盐度对ACP都有显著影响,Ca2+为100 mg/L,Mg2+为150 mg/L,盐度为30时酶活最高,Mg2+对AKP具有显著影响,在150 mg/L时酶活最高,Ca2+、盐度对SOD酶活具有显著影响,Ca2+为100 mg/L,盐度为35时酶活最高;Ca2+、Mg2+、盐度间的交互作用对体内代谢酶也有一定影响。
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| 关 键 词： | 凡纳滨对虾(Litopenaeus vannamei) Ca2+ Mg2+ 盐度 消化酶 ATP酶 免疫类酶 |
| 收稿时间： | 2011/12/12 0:00:00 |
| 修稿时间： | 2012/8/20 0:00:00 |
Effect of Ca2+, Mg2+ and salinity on metabolic enzymes of Litopenaeus vannamei |
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| ZHANG Li-tian
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DAI Xi-lin
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ZANG Wei-ling.Effect of Ca2+, Mg2+ and salinity on metabolic enzymes of Litopenaeus vannamei[J].Marine Sciences,2013,37(7):63-71. |
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| Authors: | ZHANG Li-tian DAI Xi-lin ZANG Wei-ling |
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| Institution: | ZHANG Li-tian;DAI Xi-lin;ZANG Wei-ling;College of Fisheries and Life, Shanghai Ocean University;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences; |
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| Abstract: | The L49(78) orthogonal experimental design was used to investigate the three factors Ca2+, Mg2+ and salinity in water, and seven levels of each factor were based. The L49(78) orthogonal experimental design was used to investigate the three factors Ca2+, Mg2+ and salinity in water, and two levels of each factor were based. In order to analyze the effect of Ca2+, Mg2+ and salinity in water on the growth performance and immunity of Litopenaeus vannamei, the digestive enzymes, ATP enzyme and immune enzymes were compared during the 60 d culture cycle. Results showed that Ca2+, and salinity had significant effect on digestive enzymes(P<0.05), the salinity had significant effect on pepsin, and the Ca2+ and salinity had significant effect on tgryptase. Ca2+, Mg2+ and salinity had significant effect on ATP enzymes(P<0.05), and Ca2+, Mg2+ and salinity had significant effect on Na+-K+-ATPase, with maximum activity at Ca2+ 300 mg/L, Mg2+ 500 mg/L and salinity 30. Ca2+ and Mg2+ had significant effect on Mg2+-ATPase and Ca2+ had significant effect on Ca2+-ATPase. Ca2+, Mg2+ and salinity had significant effect on immune enzymes(P<0.05), and Ca2+, Mg2+ and salinity had significant effect on ACP, with maximum activity at Ca2+ 100 mg/L, Mg2+ 150 mg/L and salinity 30. Mg2+ had significant effect on AKP with maximum activity at 150 mg/L. Ca2+ and salinity had significant effect on SOD, with maximum activity at Ca2+ 100 mg/L, salinity 35. The interaction of Ca2+, Mg2+, and salinity also had some impact on metabolic enzymes of L. vannamei. |
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| Keywords: | Litopenaeus vannamei Ca2+ Mg2+ salinity digestive enzymes ATP enzymes immune enzymes |
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