|
|||||
|
|
| 拮抗菌对病原性溶藻弧菌(Vibrio alginolyticus)粘附大黄鱼(Pseudosciaena crocea)表皮粘液的影响 | |
| 引用本文： | 郭国军,鄢庆枇,邹文政,周仁孙,陈昌生,陈 强.拮抗菌对病原性溶藻弧菌(Vibrio alginolyticus)粘附大黄鱼(Pseudosciaena crocea)表皮粘液的影响[J].海洋与湖沼,2009,40(6):738-744. |
| 作者姓名： | 郭国军 鄢庆枇 邹文政 周仁孙 陈昌生 陈 强 |
| 作者单位： | 1. 集美大学水产学院,厦门,361021;郑州牧业工程高等专科学校,郑州,450011 2. 集美大学水产学院,厦门,361021 3. 中国海洋大学水产学院,青岛,266003 |
| 基金项目： | 国家863计划项目,2007AA09Z115号;;厦门市科技项目,3502Z20073019号 |
| 摘 要： | 采用3H-TdR同位素示踪法研究了病原性溶藻弧菌和9株拮抗菌对大黄鱼表皮粘液的粘附动力学。结果表明,10株细菌的粘附作用都符合饱和粘附动力学特征;R4、R13和R32等3株拮抗菌的最大粘附量高于溶藻弧菌;9株拮抗菌的分离常数都高于溶藻弧菌;R32的亲和指数大于溶藻弧菌,其余8株拮抗菌的亲和指数都小于溶藻弧菌。测定了9株拮抗菌对病原性溶藻弧菌粘附大黄鱼表皮粘液的竞争、排斥和置换作用,结果显示:9株拮抗菌在竞争条件下都能极显著减少溶藻弧菌对表皮粘液粘附(P<0.01);在粘附排斥方面,R4、R13、J26、J28、J312、Y59等6株拮抗菌能显著排斥病原性溶藻弧菌对大黄鱼表皮粘液的粘附作用(P<0.05);在粘附置换方面,J26、J28等2株拮抗菌能极显著(P<0.01)降低病原性溶藻弧菌对大黄鱼表皮粘液的粘附量。研究结果表明,病原性溶藻弧菌对大黄鱼表皮粘液有较强的粘附作用;9株拮抗菌能够抑制溶藻弧菌对大黄鱼表皮粘液的粘附作用,其中竞争作用效果最好,排斥作用次之,置换作用的效果最差;拮抗菌对病原菌粘附的抑制效果受多种因素的影响,各菌株的粘附动力学参数有重要的影响作用。 |
| 关 键 词： | 大黄鱼 溶藻弧菌 拮抗菌 表皮粘液 粘附 |
| 收稿时间： | 2008/11/18 0:00:00 |
| 修稿时间： | 2009/1/22 0:00:00 |
INFLUENCE OF ANTAGONISTIC BACTERIA ON ADHESION OF PATHOGENIC VIBRIO ALGINOLYTICUS TO SKIN MUCUS OF PSEUDOSCAENA CROCEA |
|
| GUO Guo-Jun,YAN Qing-Pi,ZOU Wen-Zheng,ZHOU Ren-Sun,CHEN Chang-Sheng and CHEN Qiang.INFLUENCE OF ANTAGONISTIC BACTERIA ON ADHESION OF PATHOGENIC VIBRIO ALGINOLYTICUS TO SKIN MUCUS OF PSEUDOSCAENA CROCEA[J].Oceanologia Et Limnologia Sinica,2009,40(6):738-744. | |
| Authors: | GUO Guo-Jun YAN Qing-Pi ZOU Wen-Zheng ZHOU Ren-Sun CHEN Chang-Sheng and CHEN Qiang |
| Institution: | 1. Fisheries College of Jimei University;Xiamen;361021;2. Zhengzhou College of Animal Husbandry Engineering;Zhengzhou;450011;3. Fisheries College;Ocean University of China;Qingdao;266003 |
| Abstract: | The adhesion kinetics of pathogenic Vibrio alginolyticus and 9 strains of antagonistic bacteria to skin mucus of Pseudosciaena crocea were studied by using of 3H-TdR as isotope tracer. The results show that the adhesion of the 10 strains complies with saturation kinetics; the maximum number of adhered bacterial cells (em) is greater in strains R4, R13 and R32 than V. alginolyticus; the dissociation constant (kx) of all the 9 strains of antagonistic bacteria is higher than that of V. alginolyticus; the affin... |
| Keywords: | Pseudosciaena crocea Vibrio alginolyticus Antagonistic bacteria Skin mucus Adhesion |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《海洋与湖沼》浏览原始摘要信息 | |
| 点击此处可从《海洋与湖沼》下载免费的PDF全文 | |