| Cryopreservation of amphioxus (Branchiostoma belcheri) spermatozoa |
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| 作者姓名： | XU Yuyan ZHANG Shicui VERAPONG Vuthiphandchai SUBUNTITH Nimrat |
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| 作者单位： | XU Yuyan(College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China);ZHANG Shicui(College of Marine Biology, Ocean University of China, Qingdao 266003, China);VERAPONG Vuthiphandchai(Department of Aquatic Science, Burapha University, Chonburi 20131, Thailand);SUBUNTITH Nimrat(Department of Microbiology and Environmental Science Program, Faculty of Science, Burapha University, Chonburi 20131, Thailand)
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| 基金项目： | The Ministry of Science and Technology (MOST) of China |
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| 摘 要： | Amphioxus, Branchiostoma belcheri, a closest relative of vertebrates, is at a high risk of extinction due to a combination of low effective population size, altered native habitats and environmental pollution, yet little is known about cryopreservation of its gametes. This study deals with the cryopreservation of amphioxus semen. The main findings are that (1) the extender of Yao et al. is the best one among the four extenders examined; (2) the appropriate ratio of semen to extender of Yao et al. plus cryoprotectant is from 1:5 to 1:7; (3) dimethyl sulfoxide (DMSO) and methanol are the better cryoprotectants than glycerol, with DMSO giving the best results; (4) the eggs fertilized with post-thaw spermatozoa are capable of developing to at least hatching stage, and the highest hatching rate is (12.4±3.0)%. This is the first report on freezing and thawing of amphioxus spermatozoa, providing a simple and practical protocol for cryopreservation of amphioxus spermatozoa and laying a foundation for safeguarding this endangered species.
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| 关 键 词： | 冷冻保存 文昌鱼 精子 二甲基亚砜 冷冻保护剂 发展中国家 脊椎动物 种群大小 |
| 收稿时间： | 2007/10/22 0:00:00 |
| 修稿时间： | 2008/5/31 0:00:00 |
Cryopreservation of amphioxus (Branchiostoma belcheri) spermatozoa |
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| XU Yuyan,ZHANG Shicui,VERAPONG Vuthiphandchai,SUBUNTITH Nimrat.Cryopreservation of amphioxus (Branchiostoma belcheri) spermatozoa[J].Acta Oceanologica Sinica,2009,28(2):96-102. |
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| Authors: | XU Yuyan ZHANG Shicui VERAPONG Vuthiphandchai and SUBUNTITH Nimrat |
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| Institution: | 1.College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China2.College of Marine Biology, Ocean University of China, Qingdao 266003, China3.Department of Aquatic Science, Burapha University, Chonburi 20131, Thailand4.Department of Microbiology and Environmental Science Program, Faculty of Science, Burapha University, Chonburi 20131, Thailand |
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| Abstract: | Amphioxus, Branchiostoma belcheri, a closest relative of vertebrates, is at a high risk of extinction due to a combination of low effective population size, altered native habitats and environmental pollution, yet little is known about cryopreservation of its gametes. This study deals with the cryopreservation of amphioxus semen. The main findings are that (1) the extender of Yao et al. is the best one among the four extenders examined; (2) the appropriate ratio of semen to extender of Yao et al. plus cryoprotectant is from 1:5 to 1:7; (3) dimethyl sulfoxide (DMSO) and methanol are the better cryoprotectants than glycerol, with DMSO giving the best results; (4) the eggs fertilized with post-thaw spermatozoa are capable of developing to at least hatching stage, and the highest hatching rate is (12.4±3.0)%. This is the first report on freezing and thawing of amphioxus spermatozoa,providing a simple and practical protocol for cryopreservation of amphioxus spermatozoa and laying a foundation for safeguarding this endangered species. |
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| Keywords: | amphioxus Branchiostoma belcheri cryopreservation spermatozoa motility |
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