| Improvement of polyploidy induction in Eriocheir sinensis |
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| 作者姓名： | CUI Zhaoxi XIANG Jianhai ZHOU Linghu CAI Naner SONG Linsheng |
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| 作者单位： | EMBL,InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China |
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| 基金项目： | the IFS under contract，国家重点基础研究发展计划(973计划) |
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| 摘 要： | An incubating temperature of 15℃ is used to induce triploidy in Eriocheir sinensis through inhibition of the release of polar body I1, and that of 18℃ to induce tetraploidy through inhibition of the first cleavage. Flow cytometry is used to identify the ploidy in different developmental stages. For induction of triploidy in fertilized eggs in vitro, the highest induction rate observed in blastula by cytochalasin B, 6-DMAP and KC1 is 49.1%, 51.7% and 77.5%, respectively. In the KC1 treatment of pregnant crabs with the fertilized eggs, the highest triploid induction rate observed in the zoea is 85.3%. For induction of tetraploidy, the highest induction rate observed in the blastula by cytochaslasin B, 6-DMAP and KC1 is 50.3%, 54.9% and 79.8% respectively. In the KC1 treatment of pregnant crabs with the fertilized eggs, the highest induction rate in zoea is 27.3%. Through this study such difficulty as in vitro culture is overcome. Triploid zoea Eriocheir sinensis has been developed for the fh‘st time. The induction rate of tetraploid zoea has also been greatly improved.
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| 关 键 词： | 四倍性 海蟹幼虫 基因处理 三倍有机体 |
| 收稿时间： | 2003/10/11 0:00:00 |
| 修稿时间： | 2004/6/20 0:00:00 |
Improvement of polyploidy induction in Eriocheir sinensis |
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| CUI Zhaoxi,XIANG Jianhai,ZHOU Linghu,CAI Naner,SONG Linsheng.Improvement of polyploidy induction in Eriocheir sinensis[J].Acta Oceanologica Sinica,2004,23(4):725-732. |
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| Authors: | CUI Zhaoxi XIANG Jianhai ZHOU Linghu CAI Naner and SONG Linsheng |
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| Institution: | EMBL, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China |
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| Abstract: | An incubating temperature of 15 C is used to induce triploidy in Eriocheir sinensis through inhibition of the releaseof polar body II, and that of 18 C to induce tetraploidy through inhibition of the first cleavage. Flow cytometry isused to identify the ploidy in different developmental stages. For induction of triploidy in fertilized eggs in vitro, thehighest induction rate observed in blastula by cytochalasin B, 6-DMAP and KCl is 49.1%, 51.7% and 77.5%,respectively. In the KCl treatment of pregnant crabs with the fertilized eggs, the highest triploid induction rateobserved in the zoea is 85.3%. For induction of tetraploidy, the highest induction rate observed in the blastula bycytochaslasin B, 6-DMAP and KCl is 50.3%, 54.9% and 79.8% respectively. In the KCl treatment of pregnant crabswith the fertilized eggs, the highest induction rate in zoea is 27.3%. Through this study such difficulty as in vitroculture is overcome. Triploid zoea Eriocheir sinensis has been developed for the first time. The induction rate oftetraploid zoea has also been greatly improved. |
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| Keywords: | Eriocheir sinensis triploid tetraploid zoea |
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