| 饲料中豆粕含量对军曹鱼幼鱼碳、氮稳定同位素分馏的影响 |
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| 引用本文： | 张泽钊,周晖,施钢,陈刚.饲料中豆粕含量对军曹鱼幼鱼碳、氮稳定同位素分馏的影响[J].湛江海洋大学学报,2013(6):30-36. |
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| 作者姓名： | 张泽钊 周晖 施钢 陈刚 |
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| 作者单位： | [1]广东海洋大学水产学院,广东湛江524025 [2]广东省普通高校南海水产经济动物增养殖重点实验室,广东湛江524025 |
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| 基金项目： | 广东省海洋与渔业科技项目（A2009-08D03、A2010-08D04）;广东省科技计划项目（20098020308005、20108080203044,20118020307012）;国家海洋公益性行业科研专项（201205028）;广东海洋大学大学生研究创新性实验项目（CXSY200802）广东海洋大学水产学院董晓慧教授对本实验饲料设计和配制给予极大帮助,特致谢忱. |
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| 摘 要： | 配制全鱼粉饲料（以鱼粉为蛋白源）、10%豆柏饲料（用豆柏蛋白10%鱼粉）、20%豆柏饲料（用豆柏蛋白20%鱼粉）等3种等氮等能饲料，分别投喂体质量21.34± 1.54g的幼鱼24 d，研究饲料中豆柏蛋白含量对 军曹鱼幼鱼碳、氮稳定同位素分馈的影响。结果表明，随着养殖时间的延长，各饲料组军曹鱼δ13C逐渐上升，δ15N逐渐下降，虽然δ13C和δ15N变化速度上有差异，但除20%豆柏饲料组肌肉的δ15N外，其他各饲料组肌肉和 全鱼在实验结束时均已与饲料达到同位素平衡;军曹鱼肌肉和全鱼的△13^C和△15^N值均随饲料中豆柏蛋白含量 的增加而加大，△13C从4.19‰增加到5.04‰， △15N从0.18‰。增加到 2.20‰,军警鱼金鱼和肌肉的△13C和△15N 相似，但在同位素值变化速度上有差异，因此，在进行生态学营养级研究（长时间尺度）时，肌肉可以代表军警鱼的同位素特征，而在进行生理学研究（短时间尺度）时，肌肉的同位素变化则不能代表军警鱼的同位素变化。
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| 关 键 词： | 军曹鱼幼鱼 饲料 豆粕蛋白 碳稳定同位素 氮稳定同位素 同位素分馏 |
Effects of Diet Soybean Protein Concentration on Carbon and Nitrogen Isotope Fractionation of Juvenile Cobia （Rachycentron canadum L.） |
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| ZHANG Ze-zhao,ZHOU Hui,SHI Gang,CHEN Gang.Effects of Diet Soybean Protein Concentration on Carbon and Nitrogen Isotope Fractionation of Juvenile Cobia （Rachycentron canadum L.）[J].Journal of Zhanjiang Ocean University,2013(6):30-36. |
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| Authors: | ZHANG Ze-zhao ZHOU Hui SHI Gang CHEN Gang |
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| Institution: | 1. Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China; 2. Key Laboratory of Aquaculture in South China Sea for A quatic Economic Animal, Regular High Education Institute of Guangdong Province, Zhanjiang 524025, China) |
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| Abstract: | In order to investigate the effects of diet soybean protein concentration on carbon and nitrogen isotope fractionation of juvenile cobia （Rachycentron canadum L.）, three isonitrogenous and isocaloric diets were formulated： the protein source in Dl was fish meal, soybean meal protein replaced 10% and 20% fish meal in D2 and D3, and the diets were fed to juvenile cobia （21.34 ± 1.54 g） for 24 days. The results showed that, with the extending of experiment time, the △13^C of cobia increased and J15N decreased. Although the changing speed of J13C and J15N were different. The whole fish and muscle of cobia fed with different diets had reached isotope equilibrium with their diet when the feeding experiment accomplished, except for D3 muscle. The △13^C and δ15N of cobia whole fish and muscle increased with protein concentrationin soybean diet. △13^C increased from 4.19%0 and 5.04%0, and ,,115N increased from 0.18%0 to 2.20%0. The △13^C and ,,115N of cobia whole fish and muscle were similar, but the changing speed of cobia whole fish and muscle△13^C and δ15N were different. This indicated that muscle can represent the isotope character of cobia whole fish in trophic level study of ecosystem （long time scale）, but can not represent the isotope character of whole fish in physiology study （short time scale）. |
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| Keywords: | Rachycentron canadum diet soybean meal protein δ13C δ15N lsotope fractlonatlon |
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