| 六种海产虾类基因组DNA多态性的RAPD标记研究 |
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| 引用本文： | 宋林生,相建海,周岭华,张首临,刘瑞玉.六种海产虾类基因组DNA多态性的RAPD标记研究[J].海洋与湖沼,1999,30(1):62-67. |
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| 作者姓名： | 宋林生 相建海 周岭华 张首临 刘瑞玉 |
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| 作者单位： | 中国科学院海洋研究所，中国科学院海洋研究所，中国科学院海洋研究所，中国科学院海洋研究所，中国科学院海洋研究所 青岛，266071，青岛，266071，青岛，266071，青岛，266071，青岛，266071 |
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| 基金项目： | 国家自然科学基金!3947014，国家攀登计划B资助!PD-B6-5-3，中国科学院院长基金!963054 |
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| 摘 要： | 于１９９６年１０月在青岛胶州湾附近海域采集中国对虾，细巧仿对虾，周氏新对虾，鹰爪虾，脊尾白虾和脊腹褐虾等６种不同科或不同属的海产虾类，用ＲＡＰＤ技术对其基因组ＤＮＡ的多态性进行了研究。在事先优化的反应条件下，经２０个随机引物扩增，共得到２８２条多态性片段，片段长度在２３０￣２８００ｂｐ之间，根据扩增片段的共享度计算出相对遗传距离指数，然后用ＵＰＧＭＡ和ＮＪ程序进行聚类分析，结果所显示的６种虾的亲缘
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| 关 键 词： | RAPD 海产虾类 基因组DNA 遗传距离 多态性 虾 |
| 收稿时间： | 1996/12/11 0:00:00 |
| 修稿时间： | 1998/4/28 0:00:00 |
STUDIES OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS ON GENOMIC DNA POLYMORPHISM IN SIX SPECIES OF MARINE SHRIMP |
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| SONG Lin-sheng,XIANG Jian-hai,ZHOU Ling-hu,ZHANG Shou-lin and LIU Rui-yu.STUDIES OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS ON GENOMIC DNA POLYMORPHISM IN SIX SPECIES OF MARINE SHRIMP[J].Oceanologia Et Limnologia Sinica,1999,30(1):62-67. |
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| Authors: | SONG Lin-sheng XIANG Jian-hai ZHOU Ling-hu ZHANG Shou-lin and LIU Rui-yu |
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| Institution: | Institute of Oceanology,the Chinese Academy of Sciences,Qingdao,266071;Institute of Oceanology,the Chinese Academy of Sciences,Qingdao,266071;Institute of Oceanology,the Chinese Academy of Sciences,Qingdao,266071;Institute of Oceanology,the Chinese Academy of Sciences,Qingdao,266071;Institute of Oceanology,the Chinese Academy of Sciences,Qingdao,266071 |
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| Abstract: | Random amplified Polymorphic DNA (RAPD) technique was applied to study thePolymorphism of genomic DNA of six marine shrimps in different families or geniues Penaeuschinensis, Parapenaeopsis tenella, Mempenaus joyneri, Trachypenaeus curvirostris, Empalaemoncarinicauda and Crangon affinis, which were collected from Jiaoztiou Bay, Qngdao in Oct 1996.Amplifications with 20 primers under predetermined optimal reartion conditions (samples were firstbeated at 94t or 5 min. and followed by 45 cycles of l min at 94t, l Ann at 36t, and 2 minat 72t, then he1d al 72t for 10 min.) gave 282 reproducible amplified fragments ranging between230 and 2 8O0hp. The amplified fragments were scored as present (1) or absent (O) for each DNAsample and an indcx of degree of band sharing (O was calculated by using Nei and Li's matehingcoefficient method. Thc value of (1 -F) was used to evaluate genetic distances between species.The phylogenetic trees were constructed with the method of bootsttap on the basis of the presenceand absence of the an1plified Polymorphic DNA fragments, and with the methods of UPGMA andNJ on the hasis of genetic distances. The results from the thrce methods of cluster analysis aresimilar in generill, and the relationships indicated by the phylogenetic trees show the differencebetween orders, families, genus and species. The results are in good overall agreement with classicaltaxonomy. it is Suggested the random amplified Polymorphic DNA (RAPD) approaches are as usefulin providing markers for marine animal genetics as they have been for other species. |
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| Keywords: | RAPD Marine shrimp Genomic DNA Genetic distance |
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