| Preliminary study on plasma membrane fluidity of Psychrophilic Yeast Rhodotorula sp.NJ298 in low temperature |
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| 作者姓名： | 唐海田 郑洲 缪锦来 刘均铃 阚光峰 |
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| 作者单位： | College of Life Science Ludong university，Key Laboratory of Marine Bio-active Substances，First Institute of Oceanorgarphy，SOA，Key Laboratory of Marine Bio-active Substances，First Institute of Oceanorgarphy，SOA，Key Laboratory of Marine Bio-active Substances，First Institute of Oceanorgarphy，SOA，Key Laboratory of Marine Bio-active Substances，First Institute of Oceanorgarphy，SOA，Yantai 264025，China Key Laboratory of Marine Bio-active Substances，First Institute of Oceanorgarphy，SOA，Qingdao 266061，China，Qingdao 266061，China，Qingdao 266061，China，Qingdao 266061，China，Qingdao 266061，China |
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| 摘 要： | The ability of cell to modulate the fluidity of plasma membrane was crucial to the survival of microorganism at low temperature. Plasma membrane proteins, fatty acids and carotenoids profiles of Antarctic psychrophilc yeast Rhodotorula sp. NJ298 were investigated at -3 ℃, 0 ℃ and 8 ℃. The results showed that plasma membrane protein content was greater at -3 ℃ than that at 8 ℃, and a unique membrane polypeptide composition with an apparent molecular mass of 94.7 kDa was newly synthesized with SDS-PAGE analysis; GC analysis showed that the main changes of fatty acids were the percentage of unsaturated fatty acids (C18∶ 1 and C18∶ 2) and shorter chain saturated fatty acid (C10∶ 0) increased along with the decrease of the culture temperature from 8 ℃ to -3 ℃; HPLC analysis indicated that astaxanthin was the major functional carotenoids of the plasma membrane, percentage of which increased from 54.6±1.5% at 8 ℃ to 81.9±2.1% at -3 ℃. However the fluidity of plasma membrane which was determined by measuring fluorescence anisotropy was similar at -3 ℃, 0 ℃ and 8 ℃. Hence these changes in plasma membrane's characteristics were involved in the cellular cold-adaptation by which NJ298 could maintain normal plasma membrane fluidity at near-freezing temperature.
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Preliminary study on plasma membrane fluidity of Psychrophilic Yeast Rhodotorula sp. NJ298 in low temperature |
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| Tang Haitian,Zheng Zhou,Miao Jinlai,Liu Junling,Kan Guangfeng.Preliminary study on plasma membrane fluidity of Psychrophilic Yeast Rhodotorula sp.NJ298 in low temperature[J].Chinese Journal of Polar Science,2007,18(1):63-72. |
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| Authors: | Tang Haitian Zheng Zhou Miao Jinlai Liu Junling Kan Guangfeng |
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| Abstract: | The ability of cell to modulate the fluidity of plasma membrane was crucial to the survival of microorganism at low temperature. Plasma membrane proteins, fatty acids and carotenoids profiles of Antarctic psychrophilc yeast Rhodotorula sp. NJ298 were investigated at -3 ℃, 0 ℃ and 8 ℃. The results showed that plasma membrane protein content was greater at -3 ℃ than that at 8 ℃, and a unique membrane polypeptide composition with an apparent molecular mass of 94.7 kDa was newly synthesized with SDS-PAGE analysis; GC analysis showed that the main changes of fatty acids were the percentage of unsaturated fatty acids (C18∶ 1 and C18∶ 2) and shorter chain saturated fatty acid (C10∶ 0) increased along with the decrease of the culture temperature from 8 ℃ to -3 ℃; HPLC analysis indicated that astaxanthin was the major functional carotenoids of the plasma membrane, percentage of which increased from 54.6±1.5% at 8 ℃ to 81.9±2.1% at -3 ℃. However the fluidity of plasma membrane which was determined by measuring fluorescence anisotropy was similar at -3 ℃, 0 ℃ and 8 ℃. Hence these changes in plasma membrane's characteristics were involved in the cellular cold-adaptation by which NJ298 could maintain normal plasma membrane fluidity at near-freezing temperature. |
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| Keywords: | psychrophilc yeast plasma membrane fluidity fatty acid protein carotenoids cold-adaptation Rhodotorula Yeast membrane fluidity plasma study characteristics cellular maintain normal However measuring fluorescence anisotropy similar HPLC astaxanthin major functional increased decrease culture |
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