Adsorption of extracellular proteases in seawater onto filters during size fractionation |
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| Authors: | Yumiko Obayashi Satoru Suzuki |
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| Institution: | (1) Center for Marine Environmental Studies, Ehime University, Matsuyama 790-8577, Japan;(2) Division of Natural Sciences, International Christian University, Mitaka, Tokyo 181-8585, Japan |
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| Abstract: | In aquatic environments extracellular enzymes are bound to microbial cells or exist in a free and adsorbed state. Various
filters have been used to fractionate these enzymatic activities, but enzymes may be readily adsorbed onto some materials,
and such adsorption can induce errors in the estimation of enzymatic activity. In this study we examined three filters to
determine the most suitable filter for fractionation when estimating proteolytic enzyme activity in seawater. We found that
the polycarbonate Nuclepore membrane, widely used for size fractionation because of its pore-size accuracy, was the most favorable
for this purpose, even though it adsorbed slightly more enzymes than the low-protein-binding polyethersulfone membrane. We
also found that trypsin-and chymotrypsin-type enzymes were more easily adsorbed than aminopeptidases. |
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| Keywords: | Extracellular enzyme protease dissolved organic matter size fractionation filter adsorption |
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