| 商业平板式超滤膜包完整性检验：对海洋胶体生物地球化学研究的启示 |
| |
| 作者姓名： | LIN Liangshi CAI Yihu SUN Xiuwu CHEN Min |
| |
| 作者单位： | State Key Laboratory of Marine Environmental Science and College of Ocean and Earth Science, Xiamen University, Xiamen 361005, China;State Key Laboratory of Marine Environmental Science and College of Ocean and Earth Science, Xiamen University, Xiamen 361005, China;Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;State Key Laboratory of Marine Environmental Science and College of Ocean and Earth Science, Xiamen University, Xiamen 361005, China |
| |
| 基金项目： | The National Basic Research Program (973 Program) sponsored by the Ministry of Science and Technology under contract Nos 2014CB953700 and 2014CB953702; the National Natural Science Foundation of China under contract Nos 40906040 and 41276063; the Fundamental Research Funds for the Central Universities of China; the Natural Science Foundation of Fujian Province of China under contract No. 2011J01277; the Laboratory of Marine Ecosystem and Biogeochemistry of State Oceanic Administration of China under contract No. LMEB201103; the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Education of Ministry of China. |
| |
| 摘 要： | The performance and integrity of a cassette cross-flow ultrafilter(Pellicon 2, Millipore) are examined with a suite of macromolecules of different molecular masses. The retention coefficient during the cross-flow ultrafiltration experiments increases with increasing molecular mass and reaches 90% with 10 kDa dextran in both milli-Q water and ultrafiltered seawater media. Based on a 90% retention coefficient, the molecular mass cut-off for the ultrafiltration membrane is defined at 10 kDa, which is ten times(1 kDa) that rated by the manufacturer. To further validate the accuracy of the laboratory calibration, the samples from the lower Zhujiang River and the Jiulong River Estuary are ultrafiltered with the cassette ultrafiltration membrane and the colloidal organic carbon abundances in these samples are quantified with the ultrafiltration permeation model based on time series permeation subsamples. The colloidal organic carbon abundances are 5.8%–21.1% in the Jiulong River Estuary and 5.6%–11.0% in the lower Zhujiang River. These are consistent with the reported values for both estuaries as well as with the colloidal organic carbon abundances in marine environments over the coastal and open oceans with 10 kDa cut-off membranes. Therefore, these field data support the laboratory calibration result and indicate the validity of the experimental and quantification procedure adopted. The discrepancy between the nominal molecular mass cut-off and the actual pore size of the ultrafiltration membrane should be of great concern for research in colloidal and nanoparticle biogeochemistry. Careful examination of the membrane integrity should be taken during ultrafiltration experiments in order to avoid misleading molecular mass cut-off information.
|
| 关 键 词： | 海洋生物地球化学 胶体有机碳 膜完整性 超滤膜 盒式 商业 海水介质 九龙江口 |
| 收稿时间： | 2013/3/28 0:00:00 |
| 修稿时间： | 2013/10/16 0:00:00 |
On the integrity of a commercial cassette ultrafiltration membrane: implications for marine colloidal biogeochemistry |
| |
| LIN Liangshi,CAI Yihu,SUN Xiuwu,CHEN Min.On the integrity of a commercial cassette ultrafiltration membrane: implications for marine colloidal biogeochemistry[J].Acta Oceanologica Sinica,2014,33(9):109-116. |
| |
| Authors: | LIN Liangshi CAI Yihu SUN Xiuwu and CHEN Min |
| |
| Institution: | 1.State Key Laboratory of Marine Environmental Science and College of Ocean and Earth Science, Xiamen University, Xiamen 361005, China2.Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
| |
| Abstract: | The performance and integrity of a cassette cross-flow ultrafilter (Pellicon 2, Millipore) are examined with a suite of macromolecules of different molecular masses. The retention coefficient during the cross-flow ultrafiltration experiments increases with increasing molecular mass and reaches 90% with 10 kDa dextran in both milli-Q water and ultrafiltered seawater media. Based on a 90% retention coefficient, the molecular mass cut-off for the ultrafiltration membrane is defined at 10 kDa, which is ten times (1 kDa) that rated by the manufacturer. To further validate the accuracy of the laboratory calibration, the samples from the lower Zhujiang River and the Jiulong River Estuary are ultrafiltered with the cassette ultrafiltration membrane and the colloidal organic carbon abundances in these samples are quantified with the ultrafiltration permeation model based on time series permeation subsamples. The colloidal organic carbon abundances are 5.8%-21.1% in the Jiulong River Estuary and 5.6%-11.0% in the lower Zhujiang River. These are consistent with the reported values for both estuaries as well as with the colloidal organic carbon abundances in marine environments over the coastal and open oceans with 10 kDa cut-off membranes. Therefore, these field data support the laboratory calibration result and indicate the validity of the experimental and quantification procedure adopted. The discrepancy between the nominal molecular mass cut-off and the actual pore size of the ultrafiltration membrane should be of great concern for research in colloidal and nanoparticle biogeochemistry. Careful examination of the membrane integrity should be taken during ultrafiltration experiments in order to avoid misleading molecular mass cut-off information. |
| |
| Keywords: | cross-flow ultrafiltration membrane calibration colloidal organic carbon Jiulong River Estuary Zhujiang River Estuary |
| 本文献已被 CNKI 维普 SpringerLink 等数据库收录！ |
| 点击此处可从《海洋学报(英文版)》浏览原始摘要信息 |
| 点击此处可从《海洋学报(英文版)》下载免费的PDF全文 |
