Axenic culture of free-living conchocelis of<Emphasis Type="Italic">Porphyra yezoensis</Emphasis> and<Emphasis Type="Italic">Porphyra haitanensis</Emphasis> |
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| Authors: | Liu?Hui-lian Shuai?Li Email author" target="_blank">Duan?De-linEmail author Xu?Huai-shu |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China;(2) Department of Environmental Science, Qingdao University, 266071 Qingdao, China;(3) Department of Marine Biology, Ocean University of Qingdao, 266003 Qingdao, China |
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| Abstract: | After discarding marine microorganisms from conchocelis ofPorphyra yezoensis andPorphyra haitanensis, their axenic cultures were obtained through treatment with antibiotics. Antibiotic disc tests were carried out to determine
the effectiveness of each antibiotic in eliminating contaminating microorganisms. Five of 12 antibiotics tested were selected
and used to produce the axenic cultures in this study, which showed that 200 μg/mL streptomycin, 250 μg/mL penicillin, 252
μg/mL kanamycin, 30 μg/mL chloramphenicol were effective concentrations for eliminating microorganisms from conchocelis when
antibiotics were added singly step by step; whereas simultaneous combination of 150 μg/mL streptomycin, 250 (or 350) μg/mL
penicillin, 150 (or 250) μg/mL kanamycin, 70 μg/mL neomycin and 200 μg/mL chloramphenicol was also effective for producing
the axenic cultures. However, it seemed that the treatments with antibiotics applied individually were more feasible than
those will all antibiotics added at the same time. This may be due to the combined inhibiting effect of antibiotics on the
growth and development of conchocelis.
Contribution No. 2381 from the Institute of Oceanology, Chinese Academy of Sciences.
This research was subsidized by Special Funds for Major State Basie Research G1999012004 and the Foundation of the Chinese
Academy of Sciences KN95-03-04. |
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| Keywords: | axenic culture conchocelis antibiotics Porphyra |
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