| 沙生濒危物种霸王的高频植株再生研究 |
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| 引用本文： | 孙兰弟,管利萍,张颖聪,吴大利,张彦萍,侯岁稳.沙生濒危物种霸王的高频植株再生研究[J].中国沙漠,2009,29(2):312-315. |
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| 作者姓名： | 孙兰弟 管利萍 张颖聪 吴大利 张彦萍 侯岁稳 |
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| 作者单位： | 1. 兰州大学生命科学学院,干旱与草地生态教育部重点实验室,甘肃,兰州,7313000
2. 兰州大学生命科学学院,干旱与草地生态教育部重点实验室,甘肃,兰州,7313000;中国科学院近代物理研究所,甘肃,兰州,730000 |
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| 基金项目： | 中国博士后科学基金，甘肃省农业生物技术研究与应用开发项目 |
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| 摘 要： | 实验选用霸王无菌苗的茎尖、子叶、下胚轴和胚根作为材料,研究霸王不同外植体的离体培养技术。结果表明,霸王茎尖是诱导丛生芽的良好外植体,而子叶、下胚轴和胚根是诱导愈伤组织的良好外植体;霸王茎尖的最适增殖培养基是:MS+6-BA 5.0 mg·L-1+NAA 1.0 mg·L-1;最适生根培养基是:MS+IAA 1.5 mg·L-1;最适愈伤组织诱导培养基是:MS+6-BA 1.0 mg·L-1或MS+6-BA 1.0 mg·L-1+NAA 0.5 mg·L-1。沙质基质为霸王组培苗过渡的最佳基质。
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| 关 键 词： | 霸王 组织培养 外植体 |
| 收稿时间： | 2007-10-12 |
| 修稿时间： | 2007-12-3 |
High-efficiency Plant Regeneration of Zygophyllum xanthoxylon (Bunge) Maxim, an Endangering Desert Plant |
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| SUN Lan-di,GUAN Li-ping,ZHANG Ying-cong,WU Da-li,ZHANG Yan-ping,HOU Sui-wen.High-efficiency Plant Regeneration of Zygophyllum xanthoxylon (Bunge) Maxim, an Endangering Desert Plant[J].Journal of Desert Research,2009,29(2):312-315. |
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| Authors: | SUN Lan-di GUAN Li-ping ZHANG Ying-cong WU Da-li ZHANG Yan-ping HOU Sui-wen |
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| Institution: | 1.Key Laboratory of Arid and Grassland Ecology, Ministry of Education; School of Life Sciences, Lanzhou University, Lanzhou 730000, China; 2.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China |
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| Abstract: | The shoot tips, cotyledons, hypocotyls and radicles of Zygophyllum xanthoxylon (Bunge) Maxim were cultured in vitro to develop rapid propagation technology. The results showed that the shoot tips are good explants of shoot multiplication and the cotyledons, hypocotyls and radicles are good explants of callus induction; the optimum multiplication medium is MS+6-BA 5.0 mg\5L-1+NAA 1.0 mg\5L-1; the best medium for rooting is MS+IAA 1.5 mg\5L-1; the desirable callus induction medium is MS+6-BA 1.0 mg\5L-1 or MS+6-BA 1.0 mg\5L-1+NAA 0.5 mg\5L-1. Plantlets with well-developed shoots and roots were acclimatized successfully in fluviatile sand and then grew vigorously in soil, mixed with sand (1∶1). |
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| Keywords: | Zygophyllum xanthoxylon (Bunge) Maxim tissue culture explant |
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