| 半滑舌鳎促黄体激素受体基因片段的克隆及组织表达分析 |
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| 引用本文： | 陈晓燕,温海深,何峰,陈彩芳,张葭人,金国雄,史宝.半滑舌鳎促黄体激素受体基因片段的克隆及组织表达分析[J].中国海洋大学学报(自然科学版),2010,40(3). |
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| 作者姓名： | 陈晓燕 温海深 何峰 陈彩芳 张葭人 金国雄 史宝 |
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| 作者单位： | 中国海洋大学海水养殖教育部重点实验室,山东,青岛,266003 |
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| 基金项目： | 国家"十一五"科技支撑计划课题 |
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| 摘 要： | 采用巢式RT-PCR法克隆了半滑舌鳎Cynoglossus semilaevis促黄体激素受体(Luteinizing Hormonene Receptor, LHR)基因部分序列,经过BLAST比对,认为该序列与庸鲽、牙鲆、黑鲷、尼罗罗非鱼、青鳉和红鳍东方鲀的LHR基因同源性分别为97%,94%,85%,83%,82%和80%.该基因片段长度为614 bp,编码204个氨基酸,含有典型的跨膜螺旋结构区域(TM helix),属于糖蛋白激素受体(GHR)家族.组织表达分析表明,LHR基因在半滑舌鳎的卵巢、精巢、肝脏、胃、肠、鳃、心、脾、肾、头肾和脑中均有表达,表达量有所不同,以脾和肾中表达量最丰富.
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| 关 键 词： | 半滑舌鳎 促黄体激素受体 巢式RT-PCR 组织表达 |
Partial Sequence Cloning of LHR Gene in Cynoglossus semilaevis and Its Tissue Expression Analysis |
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| CHEN Xiao-Yan,WEN Hai-Shen,HE Feng,CHEN Cai-Fang,ZHANG Jia-Ren,JIN Guo-Xiong,SHI Bao.Partial Sequence Cloning of LHR Gene in Cynoglossus semilaevis and Its Tissue Expression Analysis[J].Periodical of Ocean University of China,2010,40(3). |
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| Authors: | CHEN Xiao-Yan WEN Hai-Shen HE Feng CHEN Cai-Fang ZHANG Jia-Ren JIN Guo-Xiong SHI Bao |
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| Institution: | CHEN+Xiao-Yan,WEN+Hai-Shen,HE+Feng,CHEN+Cai-Fang,ZHANG+Jia-Ren,JIN+Guo-Xiong,SHI+Bao(The+Key+Laboratory+of+Mariculture,Ministry+of+Education,Ocean+University+of+China,Qingdao+266003,China) |
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| Abstract: | Partial cDNAs encoding LHR(luteinizing hormonene receptor) gene were cloned from Cynoglossus semilaevis by nested reverse transcrip-polymerase chain reaction(nested RT-PCR).Alignment analysis showed that the sequence was 97%,94%,85%,83%,82% and 80% similar to those of Hippoglossus hippoglossus,Paralichthys olivaceus,Acanthopagrus schlegelii,Oreochromis niloticus,Oryzias latipes and Takifugu rubripes,respectively.The length of the fragment is 614 bp,and encodes a protein of about 204 amino acids,which contai... |
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| Keywords: | Cynoglossus semilaevis LHR nested RT-PCR tissue expression |
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