| 一株多环芳烃芘降解菌的鉴定及其降解特性研究 |
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| 引用本文： | 卢仕严,曹永军,招嘉佩,何 红,孙省利.一株多环芳烃芘降解菌的鉴定及其降解特性研究[J].海洋科学,2014,38(8):21-25. |
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| 作者姓名： | 卢仕严 曹永军 招嘉佩 何 红 孙省利 |
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| 作者单位： | 1. 广东海洋大学 农学院,广东 湛江,524088
2. 广东海洋大学 海洋资源与环境监测中心,广东 湛江,524088 |
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| 基金项目： | 广东省海洋渔业科技推广专项(A200899J01); 海洋公益性行业科研专项(200905005-05) |
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| 摘 要： | 从红树植物红海榄叶片中分离一株对芘具有较好降解作用的海洋细菌,命名为B11,并对其菌体特征、生长条件及降解效能等进行了系统研究。结果发现该菌株为革兰氏阴性菌,结合其生理生化特征和16S rDNA序列比对,表明此菌株属于交替单胞菌属(Alteromonas sp.)。菌株在芘的起始浓度为100 mg/L,pH为中性或偏碱性条件下,盐度35时对芘的降解作用最强。外加碳源对B11对芘的降解效果都具有一定的抑制作用,其中水杨酸的抑制作用更为显著。综合菌株B11的生长特性和降解效能,初步认为B11菌株较适合用于降解多环芳烃芘,可用于红树植物多环芳烃生态污染的原位修复。
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| 关 键 词： | 多环芳烃 海洋细菌 芘 生物降解 |
| 收稿时间： | 2013/7/28 0:00:00 |
| 修稿时间： | 2013/10/11 0:00:00 |
Isolation and characterization of a pyrene-degrading marine bacterium from mangrove (Rhizophora stylosa) |
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| LU Shi-yan
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CAO Yong-jun
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ZHAO Jia-pei
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HE Hong
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SUN Sheng-li.Isolation and characterization of a pyrene-degrading marine bacterium from mangrove (Rhizophora stylosa)[J].Marine Sciences,2014,38(8):21-25. |
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| Authors: | LU Shi-yan CAO Yong-jun ZHAO Jia-pei HE Hong SUN Sheng-li |
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| Abstract: | A predominant indigenous bacterium utilizing pyrene as a sole carbon and energy source was isolated from mangrove (Rhizophora stylosa) in Zhanjiang, China. The morphology, physiological and biochemcial characteristics, capacity of degradation were studied. The results showed that the strain was Gram-negative bacteria. Based on its characteristics mentioned above as well as the 16 S rDNA sequence analysis, the bacterium was identified as Alteromonas sp., and named B11. The best pyrene degradation condition of B11 was that the initial concentration of pyrene was 100 mg/L, pH was 7 or a little alkalinity and salinity was almost 35. Extra carbon source especially salicylic acid reduced degradation rate of pyrene by B11 strain. The results showed that strain B11 was suitable to degrade Pyrene and indigenous rehabilitation in mangrove polluted zone. |
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| Keywords: | poly-aromatic hydrocarbons (PAHs) marine bacterium pyrene microbial degradation |
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