Construction and characterization of a fosmid library for pathogenic bacterium <Emphasis Type="Italic">Vibrio anguillarum</Emphasis> |
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| Authors: | Yifan Han Zhaolan Mo Yunxiang Mao Peng Xiao Jie Li Bin Hao Dongsheng Guo |
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| Institution: | [1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Ocean University of China, Qingdao 266003, China |
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| Abstract: | Vibrio anguillarum is a common bacterial pathogen in fish.However,little is known about its pathogenic mechanism,in part,because the entire genome has not been completely sequenced.We constructed a fosmid library for V.anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage.We characterized the library by end-sequencing 50 randomly selected clones.This generated 93 sequences with a total length of 57 485 bp covering 1.4% of the whole genome.Of these sequences,58... |
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| Keywords: | end-sequencing fosmid library PCR screening Vibrio anguillarum |
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