| Tolerance of ciliated protozoan Paramecium bursaria （Protozoa,Ciliophora） to ammonia and nitrites |
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| 引用本文： | 许恒龙 宋微波 路璐 WARRENAlan.Tolerance of ciliated protozoan Paramecium bursaria （Protozoa,Ciliophora） to ammonia and nitrites[J].中国海洋与湖沼学报,2005,23(3):349-353. |
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| 作者姓名： | 许恒龙 宋微波 路璐 WARRENAlan |
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| 作者单位： | [1]TheKeyLaboratoryofMaricultureoftheEducationMinistry,OceanUniversityofChina,Qingdao266003,China／／DepartmentofBtology.LiaochengUniversity,Liaocheng252059,China [2]TheKeyLaboratoryofMaricultureoftheEducationMinistry,OceanUniversityofChina,Qingdao266003,China [3]DepartmentofZoology,NaturalHistoryMuseum,CromwellRoad,LondonSW75BD,UK,China |
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| 摘 要： | The tolerance to ammonia and nitrites in freshwater ciliate Paramecium bursaria was measured in a conventional open system. The ciliate was exposed to different concentrations of ammonia and nitrites for 2h and 12h in order to determine the lethal concentrations. Linear regression analysis revealed that the 2h-LC50 value for ammonia was 95.94 mg/L and for nitrite 27.35 mg/L using probit scale method (with 95% confidence intervals). There was a linear correlation between the mortality probit scale and logarithmic concentration of ammonia which fit by a regression equation y=7.32x-9.51 (R^2=0.98; y, mortality probit scale; x, logarithmic concentration of ammonia), by which 2 h-LC50 value for ammonia was found to be 95.50 mg/L. A linear correlation between mortality probit scales and logarithmic concentration of nitrite is also followed the regression equation y=2.86x 0.89 (R^2=0.95; y, mortality probit scale; x, logarithmic concentration of nitrite). The regression analysis of toxicity curves showed that the linear correlation between exposed time of ammonia-N LC50 valueand ammonia-N LC50 value followed the regression equation y=2 862.85e-0.08x (R^2=0.95; y, duration of exposure to LC50 value; x, LC50 value), and that between exposed time of nitrite-N LC50 value and nitrite-N LC50 value followed the regression equation y = 127.15e-0.13x (R^2=0.91; y, exposed time of LC50 value; x, LC50 value). The results demonstrate that the tolerance to ammonia in P. bursaria is considerably higher than that of the larvae or juveniles of some metozoa, e.g. cultured prawns and oysters. In addition, ciliates, as bacterial predators, are likely to play a positive role in maintaining and improving water quality in aquatic environments with high-level ammonium, such as sewage treatment systems.
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| 关 键 词： | 纤毛原生动物 氨水 亚硝酸盐 容许量 毒物学 |
| 收稿时间： | 16 February 2004 |
| 修稿时间： | 6 December 2004 |
Tolerance of ciliated protozoanParamecium bursaria (Protozoa, Ciliophora) to ammonia and nitrites |
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| Xu Henglong,Song Weibo,Lu Lu,Warren Alan.Tolerance of ciliated protozoanParamecium bursaria (Protozoa, Ciliophora) to ammonia and nitrites[J].Chinese Journal of Oceanology and Limnology,2005,23(3):349-353. |
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| Authors: | Xu Henglong Song Weibo Lu Lu Warren Alan |
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| Institution: | (1) The Key Laboratory of Mariculture of the Education Ministry, Ocean University of China, 266003 Qingdao, China;(2) Department of Biology, Liaocheng University, 252059 Liaocheng, China;(3) Department of Zoology, Natural History Museum, Cromwell Road, SW7 5BD London, UK, China |
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| Abstract: | The tolerance to ammonia and nitrites in freshwater ciliateParamecium bursaria was measured in a conventional open system. The ciliate was exposed to different concentrations of ammonia and nitrites for
2h and 12h in order to determine the lethal concentrations. Linear regression analysis revealed that the 2h-LC50 value for ammonia was 95.94 mg/L and for nitrite 27.35 mg/L using probit scale method (with 95% confidence intervals). There
was a linear correlation between the mortality probit scale and logarithmic concentration of ammonia which fit by a regression
equationy=7.32x–9.51 (R
2=0.98;y, mortality probit scale;x, logarithmic concentration of ammonia), by which 2 h-LC50 value for ammonia was found to be 95.50 mg/L. A linear correlation between mortality probit scales and logarithmic concentration
of nitrite is also followed the regression equationy=2.86x+0.89 (R
2=0.95;y, mortality probit scale;x, logarithmic concentration of nitrite). The regression analysis of toxicity curves showed that the linear correlation between
exposed time of ammonia-N LC50 value and ammonia-N LC50 value followed the regression equationy=2 862.85e
−0.08x
(R
2=0.95;y, duration of exposure to LC50 value;x, LC50 value), and that between exposed time of nitrite-N LC50 value and nitrite-N LC50 value followed the regression equationy=127.15e
−0.13x
(R
2=0.91;y, exposed time of LC50 value;x, LC50 value). The results demonstrate that the tolerance to ammonia inP. bursaria is considerably higher than that of the larvae or juveniles of some metozoa, e.g. cultured prawns and oysters. In addition,
ciliates, as bacterial predators, are likely to play a positive role in maintaining and improving water quality in aquatic
environments with high-level ammonium, such as sewage treatment systems.
This work was supported by NSFC (No. 40206021). |
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| Keywords: | toxicology ecotoxicology toxicity ammonia nitrite ciliate protozoa Paramecium bursaria |
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