| 基因定量技术在油气勘探中的应用 |
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| 引用本文： | 任春,汤玉平,何晋发,高俊阳,许科伟.基因定量技术在油气勘探中的应用[J].物探与化探,2014(5):976-980. |
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| 作者姓名： | 任春 汤玉平 何晋发 高俊阳 许科伟 |
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| 作者单位： | 中国石化石油勘探开发研究院无锡石油地质研究所,江苏无锡214151 |
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| 基金项目： | 中石化科技开发项目（P11058）;国家自然科学基金项目（41202241） |
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| 摘 要： | 针对特定功能基因发展起来的油气微生物免培养检测技术,与传统培养法相比具有快速、稳定、特异性高的特点,非常适合工业化测试。在沾化凹陷邵家洼陷陈22井区,对钻孔、剖面以及区域所采集的微生物土壤样品进行检测研究,结果显示,利用基因定量技术检测到的油气微生物异常与油气田有极好的对应关系,证明发展油气微生物分子勘探技术技术具有广阔的远景。
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| 关 键 词： | 微生物勘探 分子生物学 基因定量 油气勘探 |
The application of gene quantitative technique to oil and gas prospecting |
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| REN Chun,TANG Yu-Ping,HE Jin-Fa,GAO Jun-Yang,XU Ke-Wei.The application of gene quantitative technique to oil and gas prospecting[J].Geophysical and Geochemical Exploration,2014(5):976-980. |
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| Authors: | REN Chun TANG Yu-Ping HE Jin-Fa GAO Jun-Yang XU Ke-Wei |
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| Institution: | ( Wu＇xi Research Institute of Petroleum Geology, Research Institute of Petroleum Exploration ＆ Production, Sinopec, Wuxi 214151, China) |
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| Abstract: | Compared with traditional culture-dependent methods, culture-independent techniques based on functional genes for detec- ting oil and gas indicating microorganisms are more rapid, stable and specific, thereby suitable for commercial applications. In this study, the authors chose Chen 22 wells zone in Shaojia subsag of Zhanhua SAG as a model system. Based on the analyses of the soil samples collected from point, linear and areal surveys, the authors detected that the anomalies of gene abundance were perfectly con- sistent with the presence of the oil and gas reservoirs. Therefore, microbial molecular prospecting technique for oil and gas is very prom- ising in the future. |
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| Keywords: | microbial prospecting molecular biology gene quantitative technique oil and gas prospecting |
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