| 凡纳对虾引进亲本及其子代RFLP分析 |
| |
| 引用本文： | 李锋,刘楚吾,黄雅丽.凡纳对虾引进亲本及其子代RFLP分析[J].海洋通报,2005,24(1):31-34. |
| |
| 作者姓名： | 李锋 刘楚吾 黄雅丽 |
| |
| 作者单位： | 湛江海洋大学水产学院,广东,湛江,524025 |
| |
| 基金项目： | 广东省重点科技攻关项目(项目编号:2KB05503N)资助 |
| |
| 摘 要： | 运用 PCR-RFLP 技术分析凡纳对虾两个引进亲本群体及其各自子一代的遗传变异情况。用一对引物对这四个群体进行 mt DNA-细胞色素氧化酶I部分片段的 PCR 扩增,扩增结果为一条约 1.25 kb的条带。用 19种限制性内切酶对该扩增条带进行酶切分析,其中 Hinf I, Sty I, Taq I 这 种酶有酶切位点。用这3种 3酶进行限制性片段长度多态性 ( RFLP )分析,共获得 种限制性类型 基因型 。而且内切酶 2 ( ) Sty I在两个不同的引进亲本群体及其各自的子一代的扩增产物的酶切中,表现出明显的差异。
|
| 关 键 词： | PCR-RFLP 凡纳对虾 细胞色素氧化酶 ImtDNA |
| 文章编号： | 1001-6392(2005)01-0031-04 |
| 修稿时间： | 2003年12月8日 |
RFLP Analysis of the Primary Parent and the First Filial Generation of Pacific White Shrimp, Litopenaeus vannamei |
| |
| LI Feng,LIU Chuwu,HUANG Yali.RFLP Analysis of the Primary Parent and the First Filial Generation of Pacific White Shrimp, Litopenaeus vannamei[J].Marine Science Bulletin,2005,24(1):31-34. |
| |
| Authors: | LI Feng LIU Chuwu HUANG Yali |
| |
| Abstract: | Two groups of primary parent of Litopenaeus vannamei from Hawaii and their first filial generation have been collected for this experiment. Part of COX I gene in mtDNA was amplified by a pair of primers, and the product was a band about 1.25Kb. Polymerase chain reaction restriction fragment length polymorphisms (PCR-RFLP) technology was employed in the experiment. A total of 19 restriction endonucleases were evaluated for their usefulness to the amplified fragment of the mtDNA-COX I, and only Hinf I, Sty I, Taq I can digest it. There were two restriction types using these three endonucleases. There were some difference between two groups of parent and their filial generation. |
| |
| Keywords: | PCR-RFLP Litopenaeus vannamei COX I mtDNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
