Distribution and inducibility of P-glycoprotein in the catfish: immunohistochemical detection using the mammalian C-219 monoclonal. |
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| Authors: | K M Kleinow A M Doi A A Smith |
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| Institution: | Department of Pharmacology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. |
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| Abstract: | Mounting evidence suggests that the P-glycoprotein (pgp) efflux pump may be a modulator of bioavailability and a mode of excretion for xenobiotics. Immunohistochemistry was utilized to examine the distribution and inducibility of a pgp like protein in catfish. Immunoreactivity to the MDR C-219 monoclonal antibody was noted primarily in bile canaliculi or bile preductules of the liver, discrete areas of the extratubular region of the kidney and the columnar epithelia of the intestine. Regional differences in pgp content were noted in the intestine with the distal region containing greater pgp levels than the proximal intestine. Dietary administration of vincristine, a prototypic pgp inducer and beta-naphthoflavone an Ah agonist resulted in induction of the C-219 immunoreactivity in the liver and the distal intestine. These results are consistent in location and inducibility with pgp like proteins and support a possible relationship to xenobiotic absorption and/or excretion in the catfish. |
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