| Complete genome sequence and proteomic analysis of a thermophilic bacteriophage BV1 |
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| 作者姓名： | LIU Bin WU Suijie XIE Lianhui |
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| 作者单位： | LIU Bin(Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China);WU Suijie(Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China);XIE Lianhui(Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China) |
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| 基金项目： | The Key Natural Science Foundation of Fujian under contract No. 2007J0004; the National Natural Science Foundation of China under contract No. 40576076. |
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| 摘 要： | Viruses of thermophiles are of great interest due to their roles in gene transfer, global geochemical cycle and evolution of life on earth. However, the thermophilic bacteriophages have not been studied extensively. In this investigation, a typical bacteriophage BV1 was obtained from a thermophilic bacterium Geobacillus sp. 6k512, which was isolated from an inshore hot spring in Xiamen of China. The BV1 contained a double-stranded linear DNA of 35 055 bp, which encodes 54 open reading frames (ORFs). Interestingly, eight of the 54 BV1 ORFs shared sequence similarities to genes from human disease-relevant bacteria. Seven proteins of the purified BV1 virions were identified by proteomic analysis. Determination of BV1 functional genomics would facilitate the better understanding of the mechanism for virus-thermophile interaction.
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| 关 键 词： | 基因组序列 蛋白质组分 噬菌体 学分 高温 病毒颗粒 地球化学 开放阅读框 |
| 收稿时间： | 3/5/2009 12:00:00 AM |
| 修稿时间： | 2009/8/21 0:00:00 |
Complete genome sequence and proteomic analysis of a thermophilic bacteriophage BV1 |
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| LIU Bin,WU Suijie,XIE Lianhui.Complete genome sequence and proteomic analysis of a thermophilic bacteriophage BV1[J].Acta Oceanologica Sinica,2010,29(3):84-89. |
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| Authors: | LIU Bin WU Suijie and XIE Lianhui |
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| Institution: | 1.Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China2.Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China3.Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | Viruses of thermophiles are of great interest due to their roles in gene transfer, global geochemical cycle and evolution of life on earth. However, the thermophilic bacteriophages have not been studied extensively. In this investigation, a typical bacteriophage BV1 was obtained from a thermophilic bacterium Geobacillus sp. 6k512, which was isolated from an inshore hot spring in Xiamen of China. The BV1 contained a double-stranded linear DNA of 35 055 bp, which encodes 54 open reading frames (ORFs). Interestingly, eight of the 54 BV1 ORFs shared sequence similarities to genes from human disease-relevant bacteria. Seven proteins of the purified BV1 virions were identified by proteomic analysis. Determination of BV1 functional genomics would facilitate the better understanding of the mechanism for virus-thermophile interaction. |
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| Keywords: | thermophile bacteriophage genome sequence proteomic analysis |
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