| Characterization,Expression and Function Analysis of DAX1 Gene of Scallop （Chlamys farreri Jones and Preston 1904） During Its Gametogenesis |
| |
| 作者姓名： | LI Hailong LIU Jianguo HUANG Xiaoting WANG Dan ZHANG Zhifeng |
| |
| 作者单位： | Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao 266003, P. R. China |
| |
| 基金项目： | Acknowledgements This work was supported by the National High Technology Research and Development Program of China （863 Program） （2012AA10A402）, the Natural Science Foundation of Qingdao （11-2-4-1（10）-jch） and the Key Natural Science Foundation of Shandong Province （Z2008D02）. |
| |
| 摘 要： | DAX 1,a member of nuclear receptor superfamily,has a function in the sex determination and gonadal differentiation of several vertebrate species.However,little information about DAX1 of invertebrates is available.Here we cloned a homolog of scallop （Chlamys farreri Jones and Preston 1904） dax1,Cf-dax1,and determined its expression characteristics at mRNA and protein levels.The cDNA sequence of Cf-dax1 was 2093 bp in length,including 1404 bp open reading frame （ORF） encoding 467 amino acids.Unlike those of vertebrates,no conserved LXXLL-related motif was found in the putative DNA binding region of Cf-DAX1.Fluorescence in situ hybridization showed that Cf-dax1 located on the short arm of a pair of subtelocentric chromosomes.Tissue distribution analysis using semi-quantitative RT-PCR revealed that Cf-dax1 expressed widely in adult scallop tissues,with the highest expression level found in adductor muscle,moderate level in mantle,gill and testis,and low level in kidney,ovary and hepatopancreas.The result of quantitative real-time PCR indicated that the expression of Cf-dax1 was significantly higher （P＜0.05） in testis than in ovary at the same stage,showing a sex-dimorphic expression pattern.Furthermore,immunohistochemical detection found that Cf-DAX1 mainly located in spermatogonia and spermatocytes of testis and in oogonia and oocytes of ovary,implying that DAX1 may involve in gametogenesis of bivalves.
|
| 关 键 词： | 栉孔扇贝 配子 半定量RT-PCR 卵巢卵母细胞 cDNA序列 实时定量PCR 表征 基因 |
| 本文献已被 维普 等数据库收录！ |
|
