| Study on Cryopreservation of Porphyra yezoensis Conchocelis |
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| 作者姓名： | ZHOU Wenjun LI Yun DAI Jixun |
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| 作者单位： | The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P R. China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | Cryopreservation of Porphyra yezoensis conchocelis was conducted with cryoprotectants and a proposed pretreatment procedure and thawing methods explored. Six cryoprotectants combined by DMSO with ethylene glycol （EG）, propylene glycol （PEG）, sorbitol and sucrose were developed. The effect of prefreezing at -40℃ or -20℃ for different time durations was compared and the thawing methods were screened. It was shown that the cryoprotectant including 10% DMSO with 0.5 molL^-1 sorbitol exhibited the optimal effect. The ideal pretreatment was that conchocelis segments were stayed for 20min at -40℃ before stored in liquid nitrogen, and 40℃ water bath was proper for quick thawing. The highest recovery rate of cryopreserved P. yezoensis conchocelis reached 89.41%.
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| 关 键 词： | 壳斑藻丝状体 低温贮藏 紫菜属 海洋 |
| 收稿时间： | 15 June 2006 |
| 修稿时间： | 2006-072007-02-12 |
Study on cryopreservation of Porphyra yezoensis conchocelis |
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| ZHOU Wenjun LI Yun DAI Jixun.Study on Cryopreservation of Porphyra yezoensis Conchocelis[J].Journal of Ocean University of China,2007,6(3):299-302. |
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| Authors: | Wenjun Zhou Yun Li Jixun Dai |
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| Institution: | The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P. R. China |
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| Abstract: | Cryopreservation of Porphyra yezoensis conchocelis was conducted with cryoprotectants and a proposed pretreatment procedure and thawing methods explored. Six cryoprotectants
combined by DMSO with ethylene glycol (EG), propylene glycol (PEG), sorbitol and sucrose were developed. The effect of prefreezing
at −40°C or −20°C for different time durations was compared and the thawing methods were screened. It was shown that the cryoprotectant
including 10% DMSO with 0.5 molL−1 sorbitol exhibited the optimal effect. The ideal pretreatment was that conchocelis segments were stayed for 20 min at −40°C
before stored in liquid nitrogen, and 40°C water bath was proper for quick thawing. The highest recovery rate of cryopreserved
P. yezoensis conchocelis reached 89.41%. |
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| Keywords: | Porphyra yezoensis conchocelis cryopreservation |
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