| Efficient expression of green fluorescent protein （GFP） mediated by a chimeric promoter in Chlamydomonas reinhardtii |
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| 引用本文： | 吴锦霞,胡章立,王潮岗,黎双飞,雷安平.Efficient expression of green fluorescent protein （GFP） mediated by a chimeric promoter in Chlamydomonas reinhardtii[J].中国海洋与湖沼学报,2008,26(3):242-247. |
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| 作者姓名： | 吴锦霞 胡章立 王潮岗 黎双飞 雷安平 |
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| 作者单位： | Institute of Ecology and Environment, College of Life Sciences, Shenzhen University, Shenzhen, 518060, China |
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| 基金项目： | Supported by the High Technology Research and Development Program of China （863 Program） （No. 2005AA601010-05）, the Natural Science Foundation of Guangdong Province （No.5010492）, and the Technology Project of Shenzhen City |
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| 摘 要： | To improve the expression efficiency of exogenous genes in Chlamydomonas reinhardtii, a high efficient expression vector was constructed. Green fluorescent protein （GFP） was expressed in C. reinhardtii under the control of promoters： RBCS2 and HSP70A-RBCS2. Efficiency of transformation and expression were compared between two transgenic algae： RBCS2 mediated strain Tran-Ⅰ and HSP70A-RBCS2 mediated strain Tran-Ⅱ. Results show that HSP70A-RBCS2 could improve greatly the transformation efficiency by approximately eightfold of RBCS2, and the expression efficiency of GFP in Tran-Ⅱ was at least double of that in Tran-Ⅰ. In addition, a threefold increase of GFP in Tran-Ⅱ was induced by heat shock at 40℃. All of the results demonstrated that HSP70A-RBCS2 was more efficient than RBCS2 in expressing exogenous gene in C. reinhardtii.
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| 关 键 词： | 表达效率 绿色荧光性蛋白质 HSP70A-RBCS2 RBCS2 转换效率 |
| 收稿时间： | 4 August 2005 |
Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in <Emphasis Type="Italic">Chlamydomonas reinhardtii</Emphasis> |
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| Jinxia?Wu,Zhangli?Hu,Chaogang?Wang,Shuangfei?Li,Anping?Lei.Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii[J].Chinese Journal of Oceanology and Limnology,2008,26(3):242-247. |
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| Authors: | Jinxia Wu Zhangli Hu Chaogang Wang Shuangfei Li Anping Lei |
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| Institution: | (1) Institute of Ecology and Environment, College of Life Sciences, Shenzhen University, Shenzhen, 518060, China |
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| Abstract: | To improve the expression efficiency of exogenous genes in Chlamydomonas reinhardtii, a high efficient expression vector was constructed. Green fluorescent protein (GFP) was expressed in C. reinhardtii under the control of promoters: RBCS2 and HSP70A-RBCS2. Efficiency of transformation and expression were compared between two transgenic algae: RBCS2 mediated strain Tran-I and HSP70A-RBCS2 mediated strain Tran-II. Results show that HSP70A-RBCS2 could improve greatly the transformation efficiency by approximately eightfold of RBCS2, and the expression efficiency of GFP in Tran-II was at least double of that in Tran-I. In addition, a threefold increase
of GFP in Tran-II was induced by heat shock at 40°C. All of the results demonstrated that HSP70A-RBCS2 was more efficient than RBCS2 in expressing exogenous gene in C. reinhardtii.
Supported by the High Technology Research and Development Program of China (863 Program) (No. 2005AA601010-05), the Natural
Science Foundation of Guangdong Province (No.5010492), and the Technology Project of Shenzhen City |
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| Keywords: | Keyword" target="_blank">Keyword expression efficiency green fluorescent protein (GFP) HSP70A-RBCS2 RBCS2 transformation efficiency |
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