| 雨生红球藻多糖的提取分离及理化性质研究 |
| |
| 引用本文： | 冯以明,李广生,吴建东,王培培,赵 峡,于广利.雨生红球藻多糖的提取分离及理化性质研究[J].海洋科学,2012,36(1):17-22. |
| |
| 作者姓名： | 冯以明 李广生 吴建东 王培培 赵 峡 于广利 |
| |
| 作者单位： | 海洋药物中国海洋大学教育部重点实验室,山东青岛266003／山东省糖科学与糖工程重点实验室,山东青岛266003 |
| |
| 基金项目： | 国家自然科学基金项目(31070724; 30870506); 国家海洋局公益专项(201005024)项目; 长江学者和创新团队发展计划(IRT0944)项目资助 |
| |
| 摘 要： | 将雨生红球藻(Haematococcus pluvialis)经乙醇/乙酸乙酯(1:2,V/V)脱脂,通过冷水、热水和5%碳酸钠提取,得到3种粗多糖(HPC、HPH和HPA),通过Q-Sepharose FF阴离子交换柱进一步分级,从HPC中得到HPC1、HPC2和HPC3共3种多糖组分。运用高效液相色谱法(HPLC)、红外光谱(IR)等方法对其单糖组成、相对分子质量和基本结构特征进行分析比较。结果表明,各组分单糖组成复杂,主要含有半乳糖(Gal)、甘露糖(Man)、阿拉伯糖(Ara)、葡萄糖(Glc)、葡萄糖醛酸(GlcUA)、半乳糖醛酸(GalUA)、木糖(Xyl)、鼠李糖(Rha)、岩藻糖(Fuc)、葡萄糖胺(GlcN)和半乳糖胺(GalN),其中,Gal含量最高,大于20%。多糖HPC1、HPC2和HPC3的相对峰位分子质量分别为502.6,373.2,577.5和300.5 ku。
|
| 关 键 词： | 雨生红球藻(Haematococcus pluvialis) 多糖 理化性质 单糖组成 |
| 收稿时间： | 2011/1/27 0:00:00 |
| 修稿时间： | 2011/4/29 0:00:00 |
Extraction and isolation of polysaccharides from Haematococcus pluvialis and their physicochemical characters study |
| |
| FENG Yi-ming,LI Guang-sheng,WU Jian-dong,WANG Pei-pei,ZHAO Xia,YU Guang-li.Extraction and isolation of polysaccharides from Haematococcus pluvialis and their physicochemical characters study[J].Marine Sciences,2012,36(1):17-22. |
| |
| Authors: | FENG Yi-ming LI Guang-sheng WU Jian-dong WANG Pei-pei ZHAO Xia YU Guang-li |
| |
| Institution: | 1,2(1.Key Laboratory of Marine Drugs(Ocean University of China),Ministry of Education,Qingdao 266003,China;2.Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,Qingdao 266003,China) |
| |
| Abstract: | Three kinds of crude polysaccharides HPC,HPH and HPA were obtained from aqueous extracts of the green alga Haematococcus pluvialis with cold water,hot water and 5% Na2CO3 water solution.The crude extracts were fractionated by ion-exchange chromatography of Q-Sepharose Fast Flow column.The purified extracts were named HPC1,HPC2 and HPC3.The monosaccharide composition,relative molecular mass(Mw) and structural feature of the polysaccharides were analyzed and compared by high-performance liquid chromatography(HPLC) and Fourier Transform Infrared spectroscopy(FTIR),respectively.The results showed that the monosaccharide composition was complicated and the main components included Galactose,Mannose,Arabinose,Glucose,Glu-curonic acid,Galacturonic acid,Xylose,Rhamnose,Fucose,Glucosamine and Galactosamine.In particular,the content of Galactose was the highest and it’s higher than 20%.The relative molecular masses of polysaccharides HPC1,HPC2 and HPC3 were 502.6,373.2,577.5 and 300.5 ku,respectively. |
| |
| Keywords: | Haematococcus pluvialis polysaccharide Extraction isolation monosaccharide composition |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《海洋科学》浏览原始摘要信息 |
| 点击此处可从《海洋科学》下载免费的PDF全文 |
|
