Effects of nitrogen specification and culture method on growth of Enteromorpha prolifera |
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| Authors: | Jianping Li Weihong Zhao |
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| Institution: | LI Jianping 1,2,ZHAO Weihong 1,** 1 K ey Laboratory of Marine Ecology & Environmental Sciences,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China 2 Graduate University of Chinese Academy of Sciences,Beijing 100049,China |
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| Abstract: | Eutrophication, which is the enrichment of a water mass with inorganic and organic nutrients that support plant growth, is a key factor in stimulating phytoplankton growth. In this study, we determined the effects of various nitrogen sources, different nitrogen concentrations in the culture medium, and two culture methods on the growth of the green alga, Enteromorpha prolifera. The relationship between the specific growth rate of E. prolifera and NO 3 ? -N concentration was consistent with that estimated using the Monod equation (R 2 = 0.9713, P < 0.01). In the NO 3 ? -N medium, the maximum specific growth rate was calculated to be 0.1634/d and the semi-saturation constant was calculated to be 16.86 ??mol/L. Our results show that E. prolifera can effectively utilize NH 4 + -N, NO 3 ? -N, and NO 2 ? -N and urea-N in the range of 5 to 50 ??mol/L. NH 4 + -N was preferentially assimilated by E. prolifera, and urea-N was favorable for long-term growth. |
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| Keywords: | eutrophication N concentrations N sources culture methods E prolifera |
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