The loss of genetic diversity during captive breeding of the endangered sculpin, Trachidermus fasciatus, based on ISSR markers: implications for its conservation |
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Authors: | Xiaoxiao Bi Qiaoli Yang Tianxiang Gao Chuangju Li |
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Institution: | BI Xiaoxiao 1,YANG Qiaoli 1,GAO Tianxiang 1,**,LI Chuangju 2 1 Fisheries College,Ocean University of China,Qingdao 266003,China 2 Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou 434000,China |
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Abstract: | Inter-simple sequence repeat (ISSR) markers were used to determine the genetic variation and genetic differentiation of cultured
and wild populations of Trachidermus fasciatus, an endangered catadromous fish species in China. Six selected primers were used to amplify DNA samples from 85 individuals,
and 353 loci were detected. Relatively low genetic diversity was detected in the cultured population (the percentage of polymorphic
loci PPL = 73.80%, Nei’s gene diversity h = 0.178 2, Shannon information index I = 0.276 9). However, the genetic diversity at the species level was relatively high (PPL = 91.78%; h = 0.258 3, I = 0.398 6). The UPGMA tree grouped together the genotypes almost according to their cultured and wild origin, showing distinct
differences in genetic structure between wild and cultured populations. The pairwise F
st values confirmed significant genetic differentiation between wild and cultured samples. The cultivated population seems to
be low in genetic diversity as a result of detrimental genetic effects in the captive population. The results suggest that
ISSR markers are effective for rapid assessment of the degree of diversity of a population, thus giving important topical
information relevant to preserving endangered species. |
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Keywords: | inter-simple sequence repeat (ISSR) Trachidermus fasciatus conservation genetic diversity genetic differentiation |
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