Encystment ofChattonella antiqua in laboratory cultures |
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| Authors: | Yasuo Nakamura Tatsushi Umemori Masataka Watanabe David M Kulis Donald M Anderson |
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| Institution: | (1) Laboratory of Marine Environment, the National Institute for Environmental Studies, Onogawa, Tsukuba, 305 Ibaraki, Japan;(2) College of Agriculture and Veterinary Medicine, Nihon University, Shimouma, Setagaya, 154 Tokyo, Japan;(3) Biology Department, Woods Hole Oceanographic Institution, 02543 Woods Hole, MA, U.S.A. |
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| Abstract: | Cysts ofChattonella antiqua (Raphidophyceae) were obtained in laboratory cultures for the first time. They were formed on the surface of glass coverslips added to both P- and N- depleted mediums. The yield was 50–250 cysts cm–2. Crossing experiments showed the highest cyst production in mixed cultures, although cysts were observed in clonal cultures as well. Cysts had approximately double the DNA content of synchronously growing vegetative cells (G1 phase). The encystment process was also monitored: Under P-depleted conditions,  small cells were formed that fused to become a triangle-shaped cell which in turn changed into a cyst.These results strongly suggest that cysts are the products of fusion and are diploid. Small cells, triangle-shaped cells, and cysts are considered to be gametes, planozygotes, and hypnozygotes, respectively. Significant mortality was observed in laboratory cysts stored in the cold and dark for five months, although those remaining were capable of germination to reestablish a motile, vegetative population. |
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