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Extraction of astaxanthin from Euphausia pacific using subcritical 1, 1, 1, 2-tetrafluoroethane
Authors:HAN Yuqian  MA Qinchuan  WANG Lan  XUE Changhu
Institution:Department of Food Science and Engineering, Ocean University of China, Qingdao 266003, P.R.China
Abstract:Euphausia pacific is an important source of natural astaxanthin. Studies were carried out to assess the extractability of astaxanthin from E. pacific using subcritical 1, 1, 1, 2-tetrafluoroethane (R134a). To examine the effects of multiple process variables on the extraction yield, astaxanthin was extracted under various conditions of pressure (30?C150 bar), temperature (303?C343 K), time (10?C50 min), flow rate (2?C10 g min?1), moisture content (5.5%?C63.61%), and particle size (0.25?C0.109 mm). The results showed that the extraction yield increased with temperature, pressure, time and flow rate, but decreased with moisture content and particle size. A maximum yield of 87.74% was obtained under conditions of 100 bar, 333 K, and 30 min with a flow rate of 6 g min?1 and a moisture content of 5.5%. The substantial astaxanthin yield obtained under low-pressure conditions demonstrates that subcritical R134a is a good alternative to CO2 for extraction of astaxanthin from E. pacific.
Keywords:subcritical R134a  astaxanthin extraction  Euphausia pacific
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