| Genetic variation of Chinese and Japanese wild Pacific abalone(Haliotis discus hannai) measured by microsatellite DNA markers |
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| 作者姓名： | LI Qi KIJIMA Akihiro |
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| 作者单位： | Graduate School |
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| 基金项目： | 教育部科学技术研究项目;国家自然科学基金 |
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| 摘 要： | 1Introduction ThePacificabaloneHaliotisdiscushannaiisan importantspeciesinthecoastalfisheriesofnorthern China,andisofhighcommercialvalue.Thepro ductionofcultureseedsfortheabalonebeganin Chinaintheearly1980s.Duringthelasttwodec ades,productionofabalonehasi…
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| 关 键 词： | 遗传变异 DNA 鲍鱼 太平洋 |
| 收稿时间： | 2005-12-21 |
| 修稿时间： | 2006-04-18 |
Genetic variation of Chinese and Japanese wild Pacific abalone(Haliotis discus hannai) measured by microsatellite DNA markers |
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| LI Qi,KIJIMA Akihiro.Genetic variation of Chinese and Japanese wild Pacific abalone(Haliotis discus hannai) measured by microsatellite DNA markers[J].Acta Oceanologica Sinica,2006,25(4):146-155. |
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| Authors: | LI Qi and KIJIMA Akihiro |
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| Institution: | 1.Key Laboratory of Mariculture of the Ministry of Education, Ocean University of China, Qingdao 266003, China2.Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan |
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| Abstract: | Population differentiation and relationships among three wild populations of the Pacific abalone Haliotis discus hannai collected from coastal seas around China and Japan were estimated using microsatellite DNA analysis. The results obtained with six microsatellite loci showed a high genetic diversity for China and Japan populations. The mean number of alleles per locus ranged from 11.7 to 23.0, and the average of observed and expected heterozygosity ranged from 0.656 to 0.721, and from 0.721 to 0.793, respectively. The observed genotype frequencies at each locus were mostly in agreement with Hardy-Weinberg expectations with five exceptions. Significant differences were detected between Chinese and Japanese H. discus hannai populations Weir and Cocker-ham's fixation index(Fst) range: 0.020～0.023; Slatkin's fixation index (Rst) range: 0.016～0.044], and no obvious difference was detected between the samples of Japanese H. discus hannai populations (Fst=0.002; Rst = 0.007). The level of differentiation among populations is further evidenced by the nNeighbor-joining tree topology on which the Japanese samples were closely clustered, and the Chinese population formed a separate cluster. These results suggest that care should be taken in future management of different populations. |
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| Keywords: | Haliotis discus hannai genetic variation microsatellite DNA |
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