| 一株分离自北极海水红球菌(Rhodococcus sp.)B7740的生长条件及其粗类胡萝卜素提取方法的优化 |
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| 引用本文： | 张加蓉,杨季芳,陈福生.一株分离自北极海水红球菌(Rhodococcus sp.)B7740的生长条件及其粗类胡萝卜素提取方法的优化[J].极地研究,2012,24(2):168-177. |
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| 作者姓名： | 张加蓉 杨季芳 陈福生 |
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| 作者单位： | 1. 华中农业大学食品科技学院;2. 宁波市微生物与环境工程重点实验室;3. 浙江万里学院生物与环境学院 |
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| 基金项目： | 国家科技部国际合作重点项目，中德政府海洋双边合作项目，宁波市科技局项目 |
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| 摘 要： | 红球菌(Rhodococcus sp.)B7740分离自北冰洋海水样品，前期实验表明其能产生大量黄色素，但是该菌生物量很低。本实验通过碳源、氮源、培养温度、发酵液起始pH值、菌种接种量等单因素和正交实验优化了该菌株的培养条件，在此基础上，通过对提取剂种类及pH、提取时间、料液比等的研究，优化了色素的提取条件。实验结果表明，1L陈海水中，添加5g酵母粉，5g葡萄糖，3g乳糖，7g牛肉膏；起始pH7，培养温度25℃，菌种接种量1.0%，160r/min摇床培养7d时，生物量从6.1mg/mL上升到23.08mg/mL，提高了278.4%。以上述培养条件下得到的干菌体为材料，采用pH7的无水乙醇，当料液比1:30，浸提温度75℃，浸提1h重复提取2次时，色素含量为1160.625μg/g干菌体。对色素的分析表明，该色素为类胡萝卜素，实验结果为进一步研究极地微生物红球菌(Rhodococcus sp.)B7740产类胡萝卜素的提取工艺提供了基础。
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| 关 键 词： | 红球菌 类胡萝卜素 提取方法 |
| 收稿时间： | 2011-12-02 |
OPTIMIZATION OF GROWING CONDITIONS AND A METHOD FOR THE EXTRACTION OF CRUDE CAROTENOIDS PRODUCED BY RHODOCOCCUS SP.(STRAIN B7740)ISOLATED FROM THE ARCTIC SEA |
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| Zhang Jiarong
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Yang Jifang
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Chen Fusheng.OPTIMIZATION OF GROWING CONDITIONS AND A METHOD FOR THE EXTRACTION OF CRUDE CAROTENOIDS PRODUCED BY RHODOCOCCUS SP.(STRAIN B7740)ISOLATED FROM THE ARCTIC SEA[J].Chinese Journal of Polar Research,2012,24(2):168-177. |
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| Authors: | Zhang Jiarong Yang Jifang Chen Fusheng |
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| Abstract: | Rhodococcus sp.B7740 strain isolated from the Arctic ocean can accumulated large numbers of yellow pigments, which biomass is very low. In this study, optimal medium was obtained by singer factor and orthogonal experiment about carbon and nitrogen sources, temperature, initial pH and inoculum concentration. On this biasis, extraction conditions were optimized through about type of extraction solvent, pH, extraction time and solid to liquid ratio. The results showed that under the condition of 1L old sea water containing yeast extract5g, glucose5g, lactose3g, beef extract7g, with initial pH7, temperature 25℃, inoculum concentration1.0%, cultured 7daies on a shaker with a speed of 160r/min biomass of B7740 from 6.1mg/mL raised to 23.08mg/mL, an increase of 278.4%. Dry cells were used as material. Anhydrous ethanol with pH7 was the extracting reagent with ratio of sample to solution 1:30, temperature 75℃，extraction time 1h repeated for 2 times. In this case, the output of the pigment reached 1160.625μg/g dry sells. Analysis showed that the pigment was Carotenoid. The experiment results provided the foundation for the further study of extraction method about carotenoids produced by polar microbial Rhodococcus sp. B7740 strain. |
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| Keywords: | Rhodococcus sp carotenoids pigment extraction method |
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