Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skate Raja porasa |
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| Authors: | Fan Tingjun Jin Lingyun and Wang Xiaofeng |
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| Institution: | (1) Department of Marine Biology, College of Marine Life Sciences, Ocean University of China, 266003 Qingdao, China |
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| Abstract: | Effects of basic fibroblast growth factor (bFGF) and insulin-like growth factor II (IGF-II) on cartilage cells from proboscis
of skate,Raja porasa Günther, were investigated in this study. The cartilage cells were cultured in 20% FBS-supplemented MEM medium at 24°C. Twelve hours
after culture initiation, the cartilage cells were treated with bFGF and IGF-II at different concentration combinations. It
was found that 20 ng/ml of bFGF or 80 ng/ml of IGF-II was enough to have obvious stimulating effect on the growth and division
of skate cartilage cells. Test of bFGF and IGF-II together, revealed that 20 ng/ml of bFGF and 80 ng/ml of IGF-II together
had the best stimulating effect on the growth and division of skate cartilage cells. The cartilage cells cultured could form
a monolayer at day 7.
This work was partially supported and funded by the China Education Committee as the “Imbursement Project for Studied Abroad
Returnees”. |
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| Keywords: | state cartilage cells culture growth factors |
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