| Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones |
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| 作者姓名： | DU Zongjun ZHANG Wanyi XIA Hongjie L&#; Guoqiang CHEN Guanjun |
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| 作者单位： | DU Zongjun,CHEN Guanjun(State Key Laboratory of Microbial Technology,Shandong University,Jinan 250100,China;College of Marine Science,Shandong University at Weihai,Weihai 264209,China);ZHANG Wanyi,XIA Hongjie,L(U) Guoqiang(College of Marine Science,Shandong University at Weihai,Weihai 264209,China) |
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| 基金项目： | The State Key Laboratory of Microbial Technology under contract No. M2010-04; Independent Innovation
Foundation of Shandong University (IIFSDU); the National Natural Science Foundation of China under contract No. 40730847;
Special Non-profit Research Proj |
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| 摘 要： | A study was undertaken to investigate the heterotrophic bacterial flora associated with the sea anemones. Samples of the sea anemones Anthopleura midori were collected from the coast of Weihai and bacteria were isolated from these samples. Additionally, high numbers of viable bacteria were obtained from the celom wall and surface of anemone, the community of cultivable bacteria was very diverse. As a result of this isolation, 60 strains were obtained, 56 of them were selected for identification and characterization by 16S rRNA gene sequence analysis and limited phenotypic testing. Among these isolates, 16 strains were phylogenetically related to members of the genus Pseudoalteromonas and neighboring taxa. Other isolates included members of the genera Colwellia, Vibrio, Acinetobacter, Pseudomonas, Endozoicomonas, Roseovarius, Paracoccus, Loktanella, Leisingera, Sulfitobacter, Bacillus, Staphylococcus, Plantibacter, Microbacterium, Micrococcus, Joostella, Psychroserpens, Cellulophaga, Krokinobacter, Polaribacter and Psychrobacter. Seven potential novel species were found. Among 60 strains, 17 of them can produce proteolytic exoenzyme, 20 can produce lipolytic exoenzyme. Strain NQ8 has strong antagonistic effects on some Vibrio strains. This study demonstrates that the culturable fraction of bacteria from the sea anemones Anthopleura midori is diverse and appears to possess much potential as a source for the discovery of novel bioactive materials.
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| 关 键 词： | 异养菌 海葵 相关分析 分离 多样性 金黄色葡萄球菌 细菌菌群 基因序列分析 |
| 收稿时间： | 22 July 2004 |
| 修稿时间： | 2009/4/15 0:00:00 |
Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones |
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| DU Zongjun,ZHANG Wanyi,XIA Hongjie,L&#; Guoqiang,CHEN Guanjun.Isolation and diversity analysis of heterotrophic bacteria associated with sea anemones[J].Acta Oceanologica Sinica,2010,29(2):62-69. |
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| Authors: | DU Zongjun ZHANG Wanyi XIA Hongjie L&#; Guoqiang and CHEN Guanjun |
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| Institution: | 1.State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China;College of Marine Science, Shandong University at Weihai, Weihai 264209, China2.College of Marine Science, Shandong University at Weihai, Weihai 264209, China |
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| Abstract: | A study was undertaken to investigate the heterotrophic bacterial flora associated with the sea
anemones. Samples of the sea anemones Anthopleura midori were collected from the coast of Wei-
hai and bacteria were isolated from these samples. Additionally, high numbers of viable bacteria
were obtained from the celom wall and surface of anemone, the community of cultivable bacteria
was very diverse. As a result of this isolation, 60 strains were obtained, 56 of them were selected
for identification and characterization by 16S rRNA gene sequence analysis and limited pheno-
typic testing. Among these isolates, 16 strains were phylogenetically related to members of the
genus Pseudoalteromonas and neighboring taxa. Other isolates included members of the genera
Colwellia, Vibrio, Acinetobacter, Pseudomonas, Endozoicomonas, Roseovarius, Paracoccus, Lok-
tanella, Leisingera, Sulfitobacter, Bacillus, Staphylococcus, Plantibacter, Microbacterium, Micro-
coccus, Joostella, Psychroserpens, Cellulophaga, Krokinobacter, Polaribacter and Psychrobacter.
Seven potential novel species were found. Among 60 strains, 17 of them can produce proteolytic
exoenzyme, 20 can produce lipolytic exoenzyme. Strain NQ8 has strong antagonistic effects on
some Vibrio strains. This study demonstrates that the culturable fraction of bacteria from the sea
anemones Anthopleura midori is diverse and appears to possess much potential as a source for the
discovery of novel bioactive materials. |
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| Keywords: | Anthopleura midori Bacterial diversity 16S rRNA gene Phylogeny Identification |
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