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Time-course studies of the biotransformation enzymes in control rainbow trout when adjusting to new habitats
Authors:Sirpa Huuskonen  Tiina Rsnen  Kari Koponen  Pirjo Lindstrm-Sepp
Institution:Sirpa Huuskonen, Tiina Räsänen, Kari Koponen,Pirjo Lindström-Seppä
Abstract:Hepatic monooxygenase enzyme activities and relative cytochrome P4501A protein content were measured to evaluate the time-course alterations in rainbow trout after change in living habitat. Fish were transferred from one fish farm to the tanks of another hatchery and/or into cages kept in a lake. In the new habitats, cytochrome P450-dependent enzyme activities in rainbow trout decreased, and were at their lowest levels after two or three weeks in the summer. Later the activities partly reversed. The immunodetection of cytochrome P4501A protein expressed a similar trend as for catalytic monooxygenase activities.
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