| 透射电镜技术在好氧污泥颗粒超微结构研究中的应用 |
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| 引用本文： | 刘晓云,王琳,林跃梅.透射电镜技术在好氧污泥颗粒超微结构研究中的应用[J].中国海洋大学学报(自然科学版),2006,36(5):817-820. |
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| 作者姓名： | 刘晓云 王琳 林跃梅 |
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| 作者单位： | 中国海洋大学海洋环境与生态教育部重点实验室,山东,青岛,266003 |
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| 基金项目： | 国家自然科学基金;山东省高校实验技术项目 |
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| 摘 要： | 利用透射电镜观察研究SBR反应器中的好氧污泥颗粒。结果显示,不同成熟度的好氧污泥颗粒呈现不同的超微结构特征:成熟度高的污泥颗粒中细菌的微群落化程度高,细菌密度大,颗粒中富含构成细菌之间及微群落之间连接结构的胞外多聚物。而在成熟度低的污泥颗粒中细菌微群落化程度较低,细菌密度相对较小,构成菌间及微群落之间连接的胞外多聚物结构松散或缺失;以电镜钌红染色技术标记好氧污泥颗粒中的多糖,显示了好氧污泥颗粒中多糖的分布与结构特点,为进一步研究污泥颗粒的形成机制、多糖对污泥颗粒稳定性和沉降性能的作用机理提供了形态学依据。
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| 关 键 词： | 电镜技术 好氧污泥颗粒 胞外多聚物 多糖 |
| 文章编号： | 1672-5174(2006)05-817-04 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006-05-24 |
Application of Transmission Electron Microscope Techniques to Studying the Aerobic Sludge Granular Ultrastructure |
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| LIU Xiao-Yun,WANG Lin,LIN Yue-Mei.Application of Transmission Electron Microscope Techniques to Studying the Aerobic Sludge Granular Ultrastructure[J].Periodical of Ocean University of China,2006,36(5):817-820. |
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| Authors: | LIU Xiao-Yun WANG Lin LIN Yue-Mei |
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| Institution: | Key Lab of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, Qingdao 266003, China |
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| Abstract: | The method of ruthenium red staining combined with TEM observation was utilized successfully for research of exopolysaccharides function in aerobic granule cultured in an SBR.Experimental results revealed that the ultrastructure of matured granule was different from that of immatured granule.Comparatively,the matured granule has a higher intensity of bacteria and the bacteria tended to be aggregated together.There were large amounts of exopolysaccharides between bacteria aggregation.The reaction of ruthenium red with polysaccharides could fix the extra cellular polysaccharide and provied information on the morphological structure and distribution of exopolysaccbarides in aerobic granular sludge.The high resolution and magnification power of the TEM attributed to the perfect visualization of extracellular polymers as well as cytological details of bacterial ultrastructure.Information obtained from this method could contribute to the characterization of aerobic granule,and the mechanisms of granular formation. |
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| Keywords: | electron microscope techniques aerobic sludge granular EPS polysaccharide |
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