长牡蛎nacrein 基因的克隆、结构及进化分析 |
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引用本文: | 宋小瑞,王晓通,李 莉,张国范.长牡蛎nacrein 基因的克隆、结构及进化分析[J].海洋科学,2015,39(10):85-93. |
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作者姓名: | 宋小瑞 王晓通 李 莉 张国范 |
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作者单位: | 中国科学院 海洋研究所;中国科学院大学,中国科学院 海洋研究所,中国科学院 海洋研究所,中国科学院 海洋研究所 |
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基金项目: | 国家“973”计划资助项目(2010CB126401); 国家自然科学基金项目(40730845); 现代农业产业技术体系构建研究专项基金项目;山东省泰山学者计划及山东泰山学者攀登计划项目 |
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摘 要: | 首次在长牡蛎(Crassostrea gigas)中克隆得到一种新的贝壳基质蛋白nacrein-like protein F3的全长c DNA序列。nacrein-like protein F3基因c DNA全长1499bp,其中编码区长度为1242bp,编码一条含413个氨基酸残基的多肽链。氨基酸序列比对和结构域分析均表明其为合浦珠母贝(Pinctada fucata)nacrein的同源蛋白,含有1个保守的α-碳酸酐酶结构域,但由于重复结构域的插入,α-碳酸酐酶结构域被间隔成2个亚结构域。系统进化分析显示nacrein-like protein F3与贻贝(Mytilus californianus)nacrein-like protein进化关系最近。此外,在软体动物中,双壳纲nacrein-like proteins进化速度相对较快,推测与寒武纪时期剧烈的环境变化有关,如影响贝壳形成的海水化学变化。
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关 键 词: | 长牡蛎(Crassostrea gigas) nacrein-like protein 碳酸酐酶结构域 系统进化分析 |
收稿时间: | 2014/11/11 0:00:00 |
修稿时间: | 2014/12/28 0:00:00 |
Molecular cloning and characterization of nacrein gene in Pacific oyster (Crassostrea gigas) |
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Abstract: | The full length cDNA sequence of nacrein-like protein F3 was cloned from Pacific oyster (Crassostrea gigas) for the first time. Nacrein-like protein F3 cDNA has 1499 bp, containing a 1242 bp CDS that encod a peptide of 413 amino acids. Multiple alignment and conserved domain analysis showed that this peptide is a homolog of nacrein (Pinctada fucata), containing a carbonic anhydrase (CA) domain, which is flanked by inserted repeat sequences. Phylogenetic analysis indicated that nacrein-like protein F3 is more closely related to nacrein-like protein in Mytilus californianus than other nacrein-like proteins. Moreover, the nacrein-like proteins in bivalves seem to evolve relatively fast, which is presumably caused by the fast changing Cambrian environment, especially the marine chemical composition, which can affect shell biomineralization. |
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Keywords: | Pacific oyster (Crassostrea gigas) nacrein-like protein carbonic anhydrase domain phylogenetic analysis |
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