PAHs contamination and bacterial communities in mangrove surface sediments of the Jiulong River Estuary,China |
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| Institution: | 1. Department of Environmental Science, Zhejiang University, Hangzhou, Zhejiang 310058, China;2. Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Hangzhou, Zhejiang, 310058, China;1. College of Environmental Science and Engineering, Ocean University of China, Shandong, Qingdao 266100, China;2. Key Laboratory of Marine Environment and Ecology, Ministry of Education, Shandong, Qingdao 266100, China;1. State Key Laboratory of Marine Environment Science, Xiamen University, Xiamen 361102, China;2. Center for Marine Environmental Chemistry and Toxicology, College of the Environment & Ecology, Xiamen University, Xiamen 361102, China;3. Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen University, Xiamen 361102, China |
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| Abstract: | Sixteen sediment samples collected from eight transects in a mangrove swamp of the Jiulong River Estuary, Fujian, China were investigated for their content of polycyclic aromatic hydrocarbons (PAHs) and the biodegradation potential of the indigenous microorganisms. The bacterial community structures in the mangrove sediments and in enrichment cultures were also investigated. The results showed that the total PAHs concentration of mangrove sediments ranged from 280 to 1074 ng g?1 dry weight, that the PAHs composition pattern in the mangrove sediments was dominated by high molecular weight PAH components (4–6 rings), and that Benzoghi]perylene and Indeno1,2,3-cd]pyrene were the most dominant at different stations. Abundant PAH-degrading bacteria were found in all the stations, the values of phenanthrene-degrading bacteria ranged from 5.85 × 104 to 7.80 × 105 CFU g?1 dry weight, fluoranthene-degrading bacteria ranged from 5.25 × 104 to 5.79 × 105 CFU g?1 dry weight, pyrene-degrading bacteria ranged from 3.10 × 104 to 6.97 × 105 CFU g?1 dry weight and the benzo(a)pyrene-degrading bacteria ranged from 5.25 × 104 to 7.26 × 105 CFU g?1 dry weight. DGGE analysis of PCR-amplified 16S rDNA gene fragments confirmed that there was a remarkable shift in the composition of the bacterial community due to the addition of the different model PAH compound phenanthrene (three ring PAH), fluoranthene(four ring PAH), pyrene(four ring PAH) and benzo(a)pyrene(five ring PAH) during enrichment batch culture. Eleven strains were obtained with different morphology and different degradation ability. The presence of common bands for microbial species in the cultures and in the native mangrove sediment DNA indicated that these strains could be potential in situ PAH-degraders. |
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