PAH-degrading microbial consortium and its pyrene-degrading plasmids from mangrove sediment samples in Huian,China |
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| Institution: | 1. Graduate Program of Geology and Geochemistry, Federal University of Pará, Belém 66077-530, Brazil;2. Federal Institute of Pará, Belém 66090-020, Brazil;3. Federal Institute of Espírito Santo, Piúma 29285-000, Brazil;4. Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba 13400-000, Brazil;5. University of Reading, United Kingdom;6. LAC-UFF AMS Laboratory-Fluminense Federal University, Niteroi, Brazil;7. Department of Oceanography and Coastal Sciences, College of the Coast and Environment, Louisiana State University, Baton Rouge, LA 70803, United States of America;1. College of Ecology and Environment, Hainan University, Haikou 570228, China;2. State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China;3. School of Biology, Hainan Normal University, Haikou 571158, China;4. Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China;1. Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, PR China;2. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China;3. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China;4. Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province 350002, PR China |
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| Abstract: | PAH-degrading microbial consortium and its pyrene-degrading plasmids were enriched from the sediment samples of Huian mangroves. The consortium YL showed degrading abilities of 92.1%, 87.6%, 92.3%, and 95.8% for pyrene, fluoranthene, phenanthrene, and fluoene at 50 mg l?1 after 21 days incubation, respectively. The dynamics of pH changes in the cultures was consistent with that of PAH concentration change. Bacillus cereus Py5 and Bacillus megaterium Py6 were isolated from the consortium and observed consuming 65.8% and 33.7% of pyrene (50 mg l?1) within three weeks, respectively. The enriched Escherichia coli DH5α cells containing the plasmids of YL were demonstrated to degrade 85.7% of the original pyrene concentration at the 21st day. |
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