| 几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析 |
| |
| 引用本文： | 王波,米铁柱,吕颂辉,孙军,李荣秀,于志刚.几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析[J].海洋学报,2007,29(1):120-126. |
| |
| 作者姓名： | 王波 米铁柱 吕颂辉 孙军 李荣秀 于志刚 |
| |
| 作者单位： | 1.中国海洋大学, 山东, 青岛, 266003 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)(2001AA635090,2004AA639780)，国家重点基础研究发展规划(973)(2001CB409704)，国家自然科学基金(40306025) |
| |
| 摘 要： | 对7株赤潮原甲藻28S rDNA 5'端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28S rDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28S rDNA扩增序列长度为950~958 bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrum micans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28S rDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.
|
| 关 键 词： | 原甲藻属 28SrDNA 系统分析 |
| 文章编号： | 0253-4193（2007）01-0120-07 |
| 收稿时间： | 2005/3/22 0:00:00 |
| 修稿时间： | 2005-03-222005-11-03 |
Cloning and analysis of 28S rDNA partial sequences of several strains of Prorocentrum |
| |
| WANG Bo,MI Tie-zhu,L&#;Song-hui,SUN Jun,LI Rong-xiu and YU Zhi-gang.Cloning and analysis of 28S rDNA partial sequences of several strains of Prorocentrum[J].Acta Oceanologica Sinica (in Chinese),2007,29(1):120-126. |
| |
| Authors: | WANG Bo MI Tie-zhu L&#;Song-hui SUN Jun LI Rong-xiu and YU Zhi-gang |
| |
| Institution: | 1.Ocean University of China, Qingdao 266003, China2.Institute of Hydrobiology, Jinan University, Guangzhou 510632, China3.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China4.Shanghai Jiaotong University, Shanghai 200030, China |
| |
| Abstract: | The 28S rDNA partial sequences of several strains of Prorocentrum were cloned and sequenced,and other 14 corresponding sequences of Prorocentrum were obtained from Genbank.Then the phylogenetic trees of Prorocentrum were constructed with the methods of NJ(neighbour-joining) and ME(minimum evolution).The results demonstrated that the sequenced partial 28S rDNA of 7 strains was 950~958 bp.The phylogenetic tree based on the NJ method was similar with that based on the ME method.The sequences of the different strains within the same species are highly conservative;otherwise,the diversity among the sequences from different species was obvious.P.micans separated from the South China Sea was very different from the P.micans strains separated from other area of the world,and the distances between them were even longer than the distances between two species.The sequences from the 28S rDNA would be helpful to design species-specific probes of the phytoplankton. |
| |
| Keywords: | Prorocentrum 28S rDNA phylogenetic analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《海洋学报》浏览原始摘要信息 |
| 点击此处可从《海洋学报》下载免费的PDF全文 |
|
