| HPLC柱前衍生法测定海水中溶解态游离氨基酸 |
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| 引用本文： | 王玉君,李烈英,陆田生.HPLC柱前衍生法测定海水中溶解态游离氨基酸[J].海洋科学,1989,13(2):43-47. |
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| 作者姓名： | 王玉君 李烈英 陆田生 |
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| 作者单位： | 中国科学院海洋研究所
(王玉君，李烈英)，中国科学院海洋研究所(陆田生) |
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| 摘 要： | 本文采用反相高效液相色谱法测定海水中溶解态游离氨基酸,整个分析过程只需40 min就可使所有海水样品中的氨基酸得到很好分离,对各含6pmol的16种氨基酸混合样分析偏差在10%左右,检测极限为100fmo1,线性范围在1pmol到Inmol之间。
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| 关 键 词： | HPLC法 海水 氨基酸 溶解态 |
HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATIONOF DISSOVED FREE AMINO ACIDS BY PRECOLUMNDERIVATIZATION IN SEAWATER |
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| Wang Yujun,Li Lieying and Lu Tiansheng.HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATIONOF DISSOVED FREE AMINO ACIDS BY PRECOLUMNDERIVATIZATION IN SEAWATER[J].Marine Sciences,1989,13(2):43-47. |
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| Authors: | Wang Yujun Li Lieying and Lu Tiansheng |
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| Abstract: | This paper describes a method of reversephase high performance liquid chromatohraphy for the determination of dissolved free amino acids in seawater.The whole process takes only 40 minutes to separate the amino-acids in all the sea-water samples. The coefficient of variation of 16 kinds of amino-acids samples each containing 6 p mol is about 10%, the detection is 100 f mol, the linear range is 1p mol to 1n mol. |
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| Keywords: | Free amino acids Reversed-phase high performance liquid chromatograhy (RP-HPLC) Ophthaldialdehyde (OPA) |
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