| 乙酰微小杆菌生物合成5-甲基尿苷的研究 |
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| 引用本文： | 上官俊龙,苏境坦,郑桂兰,张文权,张贵友,谢莉萍,王洪钟.乙酰微小杆菌生物合成5-甲基尿苷的研究[J].广东海洋大学学报,2011(4). |
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| 作者姓名： | 上官俊龙 苏境坦 郑桂兰 张文权 张贵友 谢莉萍 王洪钟 |
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| 作者单位： | 清华大学生命科学学院; |
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| 基金项目： | 国家自然科学基金(40876068,21176138); 国家基础科学人才培养基金(J1030622) |
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| 摘 要： | 用乙酰微小杆菌(Exiguobacterium acetylicum)完整细胞作为酶源,催化鸟苷和胸腺嘧啶合成抗艾滋病药物关键中间体5-甲基尿苷,并对影响菌体生长和5-甲基尿苷转化率的因素进行了考察。采用紫外诱变方法获得高表达核苷磷酸化酶菌种,使5-甲基尿苷的转化率提高到了75.8%;对培养基进行了碳源和氮源的优化,结果表明,3 g/L葡萄糖、20 g/L玉米浆、4 g/L NH4Cl有利于菌体生长;通过响应曲面(Response Surface Methodology,RSM)法获得了较为合适的转化条件:0.03 g/L MnSO4加入到pH值为8.0的磷酸缓冲液,两种底物浓度均为67 mmol/L,反应温度为56℃。
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| 关 键 词： | 乙酰微小杆菌 5-甲基尿苷 紫外诱变 培养基优化 生物合成 响应曲面法 |
A Study on the Biosynthesis of 5-Methyluridine |
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| SHANGGUAN Jun-long,SU Jing-tan,ZHENG Gui-lan,ZHANG Wen-quan,ZHANG Gui-you,XIE Li-ping,WANG Hong-zhong.A Study on the Biosynthesis of 5-Methyluridine[J].Journal of Zhanjiang Ocean University,2011(4). |
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| Authors: | SHANGGUAN Jun-long SU Jing-tan ZHENG Gui-lan ZHANG Wen-quan ZHANG Gui-you XIE Li-ping WANG Hong-zhong |
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| Institution: | SHANGGUAN Jun-long,SU Jing-tan,ZHENG Gui-lan,ZHANG Wen-quan,ZHANG Gui-you,XIE Li-ping,WANG Hong-zhong(School of Life Sciences,Tsinghua University,Beijing 100084,China) |
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| Abstract: | The complete cells of Exiguobacterium acetylicum were used as the enzyme sources to catalyze guanosines and thymines for synthesizing 5-methyluridine,which is the critical intermediate of AIDS drugs.Factors affecting bacterium growth and the conversion rate of 5-methyluridine were also studied.Through UV mutagenesis of Exiguobacterium acetylicum,a strain was acquired with high expression of nucleoside phosphorylase,which improves the conversion rate of 5-methyluridine to 75.8% and also optimizes carbon and ... |
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| Keywords: | Exiguobacterium acetylicum 5-methyluridine UV mutagenesis Optimal medium Biosynthesis Response Surface Methodology |
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