Complex conductivity response to microbial growth and biofilm formation on phenanthrene spiked medium |
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| Authors: | Remy Albrecht Jean Christophe GourryMarie-Odile Simonnot Corinne Leyval |
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| Institution: | a Laboratoire des Interactions Microorganismes-Minéraux-Matière Organique dans les Sols UMR7137, Nancy Université, CNRS, Faculté des Sciences, B.P. 70239, 54506 Vandoeuvre-les-Nancy Cedex, Franceb Bureau de Recherches Géologiques et Minières, ARN, BP6009, F-45060 Orléans, Francec Laboratoire Réactions et Génie des Procédés UPR3349, Nancy Université INPL-CNRS, 1 rue Grandville, BP 20451, 54001 Nancy Cedex, France |
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| Abstract: | Several laboratory studies have recently demonstrated the utility of geophysical methods for the investigation of microbial-induced changes over contaminated sites. However, it remains difficult to distinguish the effects due to the new physical properties imparted by microbial processes, to bacterial growth, or to the development of bacterial biofilm. We chose to study the influence of biofilm formation on geophysical response using complex conductivity measurements (0.1-1000 Hz) in phenanthrene-contaminated media. Biotic assays were conducted with two phenanthrene (PHE) degrading bacterial strains: Burkholderia sp (NAH1), which produced biofilm and Stenophomonas maltophilia (MATE10), which did not, and an abiotic control. Results showed that bacterial densities for NAH1 and MATE10 strains continuously increased at the same rate during the experiment. However, the complex conductivity signature showed noticeable differences between the two bacteria, with a phase shift of 50 mrad at 4 Hz for NAH1, which produced biofilm. Biofilm volume was quantified by Scanning Confocal Laser Microscopy (SCLM). Significant correlations were established between phase shift decrease and biofilm volume for NAH1 assays. Results suggest that complex conductivity measurements, specifically phase shift, can be a useful indicator of biofilm formation inside the overall signal of microbial activity on contaminated sites. |
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| Keywords: | Bacterial biofilm Complex conductivity Microbial processes PAH |
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