| A Fast and Indirect Fluorescent Antibody Assay for the Vibrio in Large Yellow Croaker Pseudosciaena crocea (Richardson) |
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| 作者姓名： | 王军 苏永全 鄢庆枇 |
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| 作者单位： | Dept. of Oceanography & Institute of Subtropical Ocean，Dept. of Oceanography & Institute of Subtropical Ocean，Institute of Aquaculture Biotechnology & Fishery College Xiamen University，Xianmen 361005，China，Xiamen University，Xianmen 361005，China，Jimei university，Xiamen 361021，China |
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| 基金项目： | ProjectsupportedbytheNationalHigh Tech.DevelopmentplanofChina ( 863- 81 9- 0 2- 1 2 ) . |
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| 摘 要： | ＴｈｅｌａｒｇｅｙｅｌｌｏｗｃｒｏａｋｅｒＰｓｅｕｄｏｓｃｉａｅｎａｃｒｏｃｅａ (Ｒｉｃｈａｒｄｓｏｎ)ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｉｍｐｏｒｔａｎｔｃａｇｅｆａｒｍ ｉｎｇｓｐｅｃｉｅｓｉｎｓｏｕｔｈｅｒｎＣｈｉｎａ.Ｉｎｒｅｃｅｎｔｙｅａｒｓ,ｔｈｅｒａｐｉｄｄｅｖｅｌｏｐｍｅｎｔｏｆｙｅｌｌｏｗｃｒｏａｋｅｒｃｕｌｔｕｒｅｈａｓｂｅｅｎｔｒｏｕｂｌｅｄｂｙｖｉｂｒｉｏｉｎｆｅｃｔｉｏｎｒｅｑｕｉｒｉｎｇｒｅｓｏｒｔｔｏｄｒｕｇｓ,ｔｈｅａｂｕｓｅｏｆｗｈｉｃｈｄｅｔｅｒｉｏｒａｔｅｓｔｈｅｍｉ…
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A Fast and Indirect Fluorescent Antibody Assay for the Vibrio in Large Yellow Croaker Pseudosciaena crocea ( Richardson ) |
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| Abstract: | A fast and indirect fluorescent antibody assay for the Vibrio alginolyticus and V. parahaemolyticus infecting the large yellow croaker has been developed. The specific antisera for the two strains of vibrio were prepared with New Zealand rabbit and the antiserum and cross-reactive efficacy was tested by coagulation in tube. It showed that the goat anti-rabbit IgG had been labeled by fluorescence isothiocyanate (FITC). The results showed that positive reactions were 100% for the large yellow croaker Pseudosciaena crocea with typical symptom of vibrio infection, while the positive reaction to the pathogen in healthy yellow croakers reached 40%, but seemed negative for aquaculture water. The results demonstrated that this fast and indirect fluorescent antibody assay can be used not only to test the vibrio pathogen in diseased yellow croaker but also in infected animals with no symptom. |
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| Keywords: | Pseudosciaena crocea Vibrio alginolyticus V Parahaemolyticus indirect fluorescent antibody |
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