| 泥蚶(Tegillarca granosa)精氨酸激酶的基因克隆与表达 |
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| 引用本文： | 李松伟,李 晔,李成华,李太武,苏秀榕.泥蚶(Tegillarca granosa)精氨酸激酶的基因克隆与表达[J].海洋与湖沼,2011,42(1):119-123. |
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| 作者姓名： | 李松伟 李 晔 李成华 李太武 苏秀榕 |
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| 作者单位： | 1. 宁波大学生命科学与生物工程学院,宁波,315211
2. 宁波大学生命科学与生物工程学院,宁波315211;宁波城市职业技术学院,宁波315100 |
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| 基金项目： | 国家自然科学基金资助项目, 30771665 号; 长江学者和创新团队发展计划资助项目, 2007—2009; 浙江省科技厅重点科研社会发展项目, 2005C23083 号 |
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| 摘 要： | 利用PCR扩增技术,从泥蚶的cDNA文库中克隆出了精氨酸激酶(arginine kinase)基因,并进行了原核表达.精氨酸激酶是调节能量代谢的重要酶类,在无脊椎动物体内能量平衡过程中起重要作用.结果表明,泥蚶的精氨酸激酶全长1601bp,包括5'非翻译区(5'-UTR)序列332bp,3'非翻译区(3'-UTR)序列...
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| 关 键 词： | 泥蚶 精氨酸激酶 基因克隆与表达 多克隆抗体 |
| 收稿时间： | 2010/5/25 0:00:00 |
| 修稿时间： | 2010/7/12 0:00:00 |
CLONING AND OVEREXPRESSION OF TEGILLARCA GRANOSA ARGININE KINASE |
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| LI Song-Wei,LI Ye,LI Cheng-Hu,LI Tai-Wu and SU Xiu-Rong.CLONING AND OVEREXPRESSION OF TEGILLARCA GRANOSA ARGININE KINASE[J].Oceanologia Et Limnologia Sinica,2011,42(1):119-123. |
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| Authors: | LI Song-Wei LI Ye LI Cheng-Hu LI Tai-Wu and SU Xiu-Rong |
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| Institution: | LI Song-Wei1,LI Ye1,LI Cheng-Hua1,LI Tai-Wu1,2,SU Xiu-Rong1 (1. Faculty of Life Science and Biotechnology,Ningbo University,Ningbo,315211,2. Ningbo City College of Vocational Technology,315100) |
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| Abstract: | In vertebrate, arginine kinase is a phosphotransferase that plays a critical role in maintaining equilibrium in energy metabolism. We isolated full-length cDNA of arginine kinase from Tegillarca granosa. It is 1601 bp long, including 203 bp of 5' untranslated region (5'-UTR) sequence and 438 bp of 3' untranslated region (3'-UTR) sequence. It encodes 319 amino acids with arginine kinase activity in part of the amino acid sequence (CPTNLGT). And the resulting product was then cloned into the expression vector. The prokaryotic expression plasmid AK-Pet-28a(+) containing the intact ORF was constructed and the target protein was induced with IPTG. The overexpressed protein was with the expected size. This study provided information further built on the foundation in understanding the function of arginine kinase in energy metabolism and host defense. |
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| Keywords: | Tegillarca granosa Arginine kinase Genic cloning and expression Polyclonal antibody |
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