| 海洋生物样品中硒的氢化物发生-原子荧光分析(HG-AFS) |
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| 引用本文： | 张朝辉.海洋生物样品中硒的氢化物发生-原子荧光分析(HG-AFS)[J].中国海洋大学学报(自然科学版),2001,31(3):375-381. |
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| 作者姓名： | 张朝辉 |
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| 作者单位： | 青岛海洋大学水产学院, |
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| 摘 要： | 采用硼氢化钠还原法将试样中硒还原为挥发性硒化氢 ,氩为载气将硒化氢吹出并导入火焰原子化器进行原子荧光测定。以正交设计法对该还原反应、氢化物转移、原子化及原子荧光测定条件等进行了详细研究。实验结果表明 ,在佳化条件下 ,该法检出限为 0 .0 72μ g/ dm3,相对标准偏差为 2 .0 %。标准曲线表明 ,在 0 .0 0 0～ 1.0 0 0μ g/ dm3Se浓度范围内 ,有理想的线性关系。本法测定海洋生物样品硒的回收率为 95.8%～ 10 2 .4 %。
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| 关 键 词： | 氢化物发生-原子荧光法 硒 海洋生物样品 |
| 文章编号： | 1001-1862(2001)03-375-07 |
| 修稿时间： | 2000年11月14 |
Determination of Selenium in Marine Organism Samples by HG-AFS |
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| Zhang Zhaohui.Determination of Selenium in Marine Organism Samples by HG-AFS[J].Periodical of Ocean University of China,2001,31(3):375-381. |
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| Authors: | Zhang Zhaohui |
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| Abstract: | The method is based on the reduction of Selenium to volatile SeH2 which is bubbled out by a carrier gas of pure argon and then swept to an Ar-H2 flame quartz atomizer to measure its atomic fluorescence. The hydride generation, transportation, atomisation and some instrumental Parameters were studied by a kind of orthogonal design. The optimum conditions selected are as follows: reactive acidity, 20% HCl; the amount of NaBH4, 0.7% 4.9ml in 5ml sample; the gas flow of Argon, 600m/min; atomize temperature, 200; lamp current, 100 mA; negative voltage, 300V. The detection limit is 0.072μg/dm3. The calibration curve shows an ideal linearity in the concentration range of 0.000～0.1000μg/dm3 Se, The recovery is 95.8%～102.4%. |
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| Keywords: | hydride generation atomic fluorescene spectrotometry (HG AFS) selenium marine organism sample |
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