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SUBUNIT COMPOSITION AND CHROMOPHORE CONTENT OF R-PHYCOERYTHRIN FROM PORPHYRA HAITANENSIS
作者姓名:高洪峰  纪明侯  曹文达
作者单位:Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071
摘    要:R-phycoerythrin from Porphyra haitanensis exists in two aggregaion states with different molecularweights. A more highly aggregated form, RPE I,Was chromatographed on Bio-Rex 70 column with ureasolution (pH 3.0) as eluent, and the molecular weights of the 3 subunits (α,β,γ) obtained were determinedon SDS-PAGE at 18000, 19200 and 30000, respatively. α subunit carried two phycoerythrobilin (PEB),β subunit, three PEB and one phycourobilin (PUB),γ subunit, one PEB and three PUB chromophores.The molar ratio of α,β, and γ subunits of RPE I wn 6:6:1, and their subunit composition was con-fired to be (αβ)_6γon account of the molecular weight of RPE I, 232000.A lower aggregated form, RPE Ⅱ, contained α and β subunits similar to those of RPE I, but itssubunit composition was the (αβ) monomer of RPE.


SUBUNIT COMPOSITION AND CHROMOPHORE CONTENT OF R-PHYCOERYTHRIN FROM PORPHYRA HAITANENSIS
GAO Hong-feng JI Ming-hou CAO Wen-da.SUBUNIT COMPOSITION AND CHROMOPHORE CONTENT OF R-PHYCOERYTHRIN FROM PORPHYRA HAITANENSIS[J].Chinese Journal of Oceanology and Limnology,1996(1).
Authors:GAO Hong-feng JI Ming-hou CAO Wen-da
Abstract:
Keywords:Porphyra haitanensis  R-phycoerythnn  subunit  chromophote
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