| Intragenic and intergenic sequences regulating the expression of the 5'-to-5' linked adult αand β-globin genes from large yellow croaker Pseudosciaena crocea |
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| 作者姓名： | CHU Wuying YU Lian QIAN Ronghu MENG Tao ZHOU Ruixue FU Guihong CHEN Ji ZHANG Jianshe |
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| 作者单位： | CHU Wuying(Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China;Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China);YU Lian,QIAN Ronghua(Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China);MENG Tao,ZHOU Ruixue,FU Guihong,CHEN Jia,ZHANG Jianshe(Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China) |
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| 基金项目： | 国家自然科学基金，the Major Program of Zhejiang Province of China under contract |
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| 摘 要： | One adult α-globin gene and one β-globin gene have been cloned from the large yellow croaker Pseudosciaena crocea. Linkage analysis indicated that the α- and β-globin genes were oriented head-to-head relative to each other. To identify the regulatory elements present in the intergenic and intragenic regions of the globin complex, the intergenic region alone or together with the β-globin gene first intron was cloned into the luciferase-reporter vector pGL3-Basic respectively, and the chimeric constructs were tran- siently transfected into Vero cells and primary fish erythrocytes. The intergenic region cannot support the high-level expression of luciferase. However, the promoter activity of the intergenic region was strongly stimulated by the positive regulatory elements （PRE） located in the β-globin gene intron 1. Thus, it is proposed that the intergenic promoters and intragenic PRE were necessary for the effective expression of the linked α- and β-globin genes.
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| 关 键 词： | 噪声 海洋学 物理性质 声速 |
| 收稿时间： | 2007/1/23 0:00:00 |
| 修稿时间： | 2007/11/12 0:00:00 |
Intragenic and intergenic sequences regulating the expression of the 5'-to-5' linked adult α- and β-globin genes from large yellow croaker Pseudosciaena crocea |
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| CHU Wuying,YU Lian,QIAN Ronghu,MENG Tao,ZHOU Ruixue,FU Guihong,CHEN Ji,ZHANG Jianshe.Intragenic and intergenic sequences regulating the expression of the 5''-to-5'' linked adult α- and β-globin genes from large yellow croaker Pseudosciaena crocea[J].Acta Oceanologica Sinica,2008,27(5):126-133. |
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| Authors: | CHU Wuying YU Lian QIAN Ronghu MENG Tao ZHOU Ruixue FU Guihong CHEN Jia and ZHANG Jianshe |
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| Institution: | 1.Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China;Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China2.Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China3.Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China |
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| Abstract: | One adult α-globin gene and one β-globin gene have been cloned from the large yellow croaker Pseudosciaena crocea. Linkage analysis indicated that the α- and β-globin genes were oriented head-to-head relative to each other. To identify the regulatory elements present in the intergenic and intragenie regions of the globin complex, the intergenic region alone or together with the β-giobin gene first intron was cloned into the lucfferase-reporter vector pGL3-Basic respectively, and the chimeric constructs were transiently transfected into Vero cells and primary fish erythrocytes. The intergenic region cannot support the high-level expression of luciferase. However, the promoter activity of the intergenic region was strongly stimulated by the positive regulatory elements (PRE) located in the β-globin gene intrun 1. Thus, it is proposed that the intergenic promoters and intragenic PRE were necessary for the effective expression of the linked α- and β-globin genes. |
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| Keywords: | globin intergenic intragenic intron large yellow croaker promoter regulation |
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