| 外源神经递质5-羟色胺(5-HT)和多巴胺(DA)调控三疣梭子蟹争斗行为的初步研究 |
| |
| 引用本文： | 吴波,张顺,张成,母昌考,徐善良,王丹丽.外源神经递质5-羟色胺(5-HT)和多巴胺(DA)调控三疣梭子蟹争斗行为的初步研究[J].海洋与湖沼,2022,53(1):168-175. |
| |
| 作者姓名： | 吴波 张顺 张成 母昌考 徐善良 王丹丽 |
| |
| 作者单位： | 宁波大学海洋学院 浙江宁波315211;宁波大学海洋学院 浙江宁波315211;海洋生物技术教育部重点实验室 浙江宁波315211 |
| |
| 基金项目： | 浙江省重大科技专项,2019C02057号;国家自然科学基金项目,42076087号。 |
| |
| 摘 要： | 影响甲壳类动物争斗行为的因素包括性别差异、体型和生殖状态等,神经递质对甲壳动物的争斗行为也具有调控作用.为探索三疣梭子蟹在养殖和繁殖过程中由于争斗行为受损或死亡的行为机制,采用双抗体夹心法测定争斗前后三疣梭子蟹血淋巴中5-HT和DA的含量,并分析了争斗前后肌肉、胸神经节、肝胰腺三种组织中5-HT和DA受体基因表达量变化...
|
| 关 键 词： | 三疣梭子蟹 争斗行为 5-HT/DA 5-HT/DA受体 |
| 收稿时间： | 2021/6/8 0:00:00 |
| 修稿时间： | 2021/8/13 0:00:00 |
PRELIMINARY STUDY ON THE REGULATION OF AGONISTIC BEHAVIOR OF PORTUNUS TRITUBERCULATUS BY EXOGENOUS 5-HT AND DA |
| |
| WU Bo,ZHANG Shun,ZHANG Cheng,MU Chang-Kao,XU Shan-Liang,WANG Dan-Li.PRELIMINARY STUDY ON THE REGULATION OF AGONISTIC BEHAVIOR OF PORTUNUS TRITUBERCULATUS BY EXOGENOUS 5-HT AND DA[J].Oceanologia Et Limnologia Sinica,2022,53(1):168-175. |
| |
| Authors: | WU Bo ZHANG Shun ZHANG Cheng MU Chang-Kao XU Shan-Liang WANG Dan-Li |
| |
| Institution: | (School of Marine Science,Ningbo University,Ningbo 315211,China;Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211,China) |
| |
| Abstract: | To explore the behavioral mechanism of damage or death of Portunus trituberculatus due to agonistic behavior during culture and reproduction,contents of 5-HT and DA in hemolymph of P.trituberculatus before and after fight were determined by double antibody sandwich method,and the changes of 5-HT and DA receptor gene expression in muscle,thoracic ganglion,and hepatopancreas before and after fight were analyzed.Meanwhile,the difference of agonistic behavior and the changes of 5-HT and DA receptor gene expression in the three tissues were recorded and analyzed by injecting exogenous 5-HT and DA.Results show that,after fighting,the content of 5-HT in hemolymph of P.trituberculatus increased significantly,while the content of DA decreased significantly(P<0.05).Injection of 10;-mol 5-HT could significantly reduce the frequency and duration of agonistic behavior(P<0.05),while injection of 10;-mol DA could significantly increase the frequency and duration of agonistic behavior of P.trituberculatus(P<0.05).The expression of 5-HT and DA receptor in muscle and thoracic ganglion changed significantly before and after fight,and different receptors did not show the same trend in the same tissue.Exogenous 5-HT could also regulate the expression of DA1 A and DA2 receptors,and exogenous DA could regulate the expression of 5-HT1 B,5-HT2 B,and 5-HT7 receptors.Therefore,5-HT can inhibit the agonistic behavior of P.trituberculatus,while DA can promote the agonistic behavior.In addition,5-HT and DA receptor genes can participate in the regulation of agonistic behavior of P.trituberculatus through 5-HT/DA,interactively. |
| |
| Keywords: | Portunus trituberculatus agonistic behavior 5-HT/DA 5-HT/DA receptor |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《海洋与湖沼》浏览原始摘要信息 |
| 点击此处可从《海洋与湖沼》下载免费的PDF全文 |
|
