| 胶质芽孢杆菌的胞外分泌物与细菌的解钾作用 |
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| 引用本文： | 杜叶,周雪莹,连宾.胶质芽孢杆菌的胞外分泌物与细菌的解钾作用[J].地学前缘,2008,15(6):107. |
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| 作者姓名： | 杜叶 周雪莹 连宾 |
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| 基金项目： | 国家高技术研究发展计划"863"课题
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| 摘 要： | 研究了胶质芽孢杆菌(Bacillus mucilaginosus)KO1菌株在不同培养条件下,其荚膜多糖含量随时间的变化以及菌株在有氮、无氮培养基中的解钾作用。结果表明,尽管该菌在无氮培养条件下的菌体数量远小于有氮培养条件,但无氮培养条件下所提取的细菌多糖多于在有氮条件下培养所提取的细菌多糖;胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的无氮培养基中生长可形成大量多糖,采用丙酮法进行细菌培养液多糖提取,发现细菌培养的第3天所提多糖含量最高;胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的有氮培养基中生长亦可形成较多的多糖,且在细菌培养的第4天所提多糖含量最高;胶质芽孢杆菌在有氮条件下对含钾矿物的释钾率高于在无氮条件下的释钾率,这可能与该菌在有氮条件下生长更快、可产生较多菌体细胞有关。分析表明,该菌在有氮条件下产生的胞外糖蛋白高于在无氮条件下产生的胞外糖蛋白,蛋白质在细菌分解转化含钾矿物过程中可能发挥重要作用。
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| 关 键 词： | 胶质芽孢杆菌 多糖 解钾 胞外分泌物 |
The extracellular secretion of Bacillus mucilaginosus and its capability of releasing potassium from potassium-bearing minerals |
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| Du Ye,Zhou Xueying,Lian Bin.The extracellular secretion of Bacillus mucilaginosus and its capability of releasing potassium from potassium-bearing minerals[J].Earth Science Frontiers,2008,15(6):107. |
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| Authors: | Du Ye Zhou Xueying Lian Bin |
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| Abstract: | In this paper,we have studied the content of the capsular polysaccharides of Bacillus mucilaginosus KO1 and the capability of the bacterial potassium-releasing from K-feldspar and biotite in the medium containing nitrogen or free of nitrogen.The results show that though the strain is growing slower in the nitrogen-free medium than in the nitrogen-containing medium,the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium.The capsular polysaccharides produced by the strain in the culture which contains different mineral powders will reach the highest content on the third or the fourth day in its growing period.The strain's capability of releasing potassium from k-feldspar and biotite in the nitrogen-containing medium is higher than that in the nitrogen-free medium because in the nitrogen-free medium,the strain and its production of glucoprotein are less than those in the nitrogen-containing medium.We propose that the protein secreted by the bacterial may play an important role in the process of potassium-releasing from potassium-bearing minerals. |
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| Keywords: | Bacillus mucilaginosus polysaccharides potassium-releasing extracellular secretion |
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