| 雪卡毒素的提取纯化方法初步研究 |
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| 引用本文： | 闫鸿鹏,张彩霞,赵肃清,张焜.雪卡毒素的提取纯化方法初步研究[J].海洋科学进展,2012,30(3):408-415. |
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| 作者姓名： | 闫鸿鹏 张彩霞 赵肃清 张焜 |
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| 作者单位： | 广东工业大学 |
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| 基金项目： | 国家自然科学基金——雪卡毒素单链抗体的量子点标记及滤量分析方法研究(20872020)和单链抗体绿色荧光触合蛋白为基础的雪卡毒素检测方法研究(20672023);广东省自然科学基金——量子点标记雪卡毒素单链抗体及痕量分析方法研究(8251009001000005) |
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| 摘 要： | 雪卡毒素(ciguatoxin)是一类具有神经毒性的海洋藻类毒素,高纯度雪卡毒素是开展其相关研究的物质基础。以石斑鱼肝脏为原料,对其脂溶性粗提物经Florisil柱和Sephadex LH-20柱纯化后,用高效液相色谱/质谱(HPLC/MS)和细胞毒性方法对其进行鉴定,证明为太平洋雪卡毒素(P-CTX-1)。HPLC鉴定分析提取物与太平洋雪卡毒素(P-CTX-1)具有相同的保留时间。HPLC/MS检测表明纯化样品在m/z=1 111.3和m/z=1 133.0处出现雪卡毒素M+H]+峰和M+Na]+峰,与已有的P-CTX-1分子离子峰完全吻合。四甲基偶氮唑蓝法(MTT)细胞毒性试验证明了雪卡毒素样品对人神经母细胞瘤(SK-N-SH)具有毒性作用,毒素剂量与细胞生长抑制率成线性关系。细胞毒性试验进一步确认该提取物为雪卡毒素,且纯度较高。
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| 关 键 词： | 雪卡毒素 提取 纯化 HPLC/MS MTT |
Preliminary Study on Extraction and Purification of Ciguatoxin |
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| YAN Hong-peng,ZHANG.Preliminary Study on Extraction and Purification of Ciguatoxin[J].Advances in Marine Science,2012,30(3):408-415. |
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| Authors: | YAN Hong-peng ZHANG |
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| Institution: | Cai-xia,ZHAO Su-qing,ZHANG Kun(Guangdong University of Technology,Guangzhou 510006,China) |
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| Abstract: | Ciguatoxin is a kind of neurotoxic marine algal toxins.However,ciguatoxin in high purity is the material foundation for related research.In this paper,ciguatoxin was extracted from Epinephelussp liver and purified by Florisil and Sephadex LH-20chromatography.Furthermore,this ciguatoxin was identified as Pacific ciguatoxin(P-CTX-1)by high-performance liquid chromatography/mass spectrometry(HPLC/ MS)and cytotoxicity assay.This ciguatoxin has the same retention time comparing to the reference compound P-CTX-1in HPLC.The mass spectra of this ciguatoxin showed the mass peaks of 1 111.3(M+ H]+)and 1 133.0(M+Na]+),which was fully consistent with the reported mass peaks of P-CTX-1.The cytotoxicity of ciguatoxin on the human neuroblastoma cell(SK-N-SH)was determined by MTT assay,and the results indicated a linear dose-dependent inhibition effect of ciguatoxin on proliferation of the cells.The cytotoxicity assay further confirmed it as ciguatoxin in high purity. |
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| Keywords: | ciguatoxin extraction purification HPLC/MS MTT |
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